HPLC同时测定胃力片中柚皮苷、橙皮苷和新橙皮苷的含量  

Determination of narigin,hesperidin and neohesperidin in Weili Tablet by HPLC

作　　者：徐海燕[1] 金艺[2] 张红霞[2] 赵怀清[2] 

机构地区：[1]沈阳药科大学高等职业技术学院,辽宁沈阳110026 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中成药》2010年第8期1355-1357,共3页Chinese Traditional Patent Medicine

摘　　要：目的:建立胃力片(半夏、龙胆、枳实等)中柚皮苷、橙皮苷和新橙皮苷的含量测定方法。方法:采用HPLC法,以DiamonsilC18柱(250mm×4.6mm,5μm)分离,乙腈-1%冰醋酸溶液(19∶81,v/v)为流动相洗脱,流速:1.0mL/min,检测波长:283nm。结果:柚皮苷、橙皮苷和新橙皮苷分别在6.0～60.0μg/mL、2.24～22.4μg/mL和10.2～102.0μg/mL范围内质量浓度与峰面积呈良好的线性关系,相关系数分别为0.9997、0.9997和0.9993,平均回收率分别为100.2%(RSD=1.5%,n=9)、98.8%(RSD=2.9%,n=9)和97.1%(RSD=0.86%,n=9)。结论:本法重复性好、灵敏度高、定量准确,可作为胃力片质量控制方法之一。AIM： To develop an HPLC method for determining narigin,hesperidin and neohesperidin in Weili Tablet（Rhizoma Pinelliae,Radix et Rhizoma Gentianae,Fructus Aurantii immaturus etc. ）. METHODS： The analysis was carried out with a Diamonsil C18（4. 6 mm × 250 mm,5 μm）column and the mobile phase of acetoni-trile-1% glacical acetic acid solution（19：81,v/v）. The flow rate was 1. 0 mL/min and column temperature was 30 ℃. The detection wavelength was set at 283 nm. RESULTS： The calibration curve was linear within the range of 6. 0-60. 0 μg/mL,2. 24-22. 4 μg/mL and 10. 2-102. 0 μg/mL for narigin,hesperidin and neohesperidin,respec-tively. The average recoveries were 100. 2% ,98. 8% and 97. 1% for narigin,hesperidine and neohesperidine,re-spectively. CONCLUSION： The method is reproducible,sensitive and accurate for determination of narigin,hes-peridin and neohesperidin in Weili Tablet.

关 键 词：胃力片 柚皮苷 橙皮苷 新橙皮苷 HPLC 

分 类 号：R927.2[医药卫生—药学]