HPLC测定黄芪蜜炙前后毛蕊异黄酮和芒柄花素的含量  

Simultaneous determination of calycosin and fermononetin in Radix Astragali and its fried product with honey by HPLC

作　　者：田圣志[1] 杨玉涛[1] 张振凌[1] 常良[1] 

机构地区：[1]河南中医学院,河南郑州450008

出　　处：《中成药》2010年第8期1365-1368,共4页Chinese Traditional Patent Medicine

摘　　要：目的:比较黄芪蜜炙前后毛蕊异黄酮和芒柄花素的含量变化。方法:采用Waters SunfireTM C18色谱柱(5μm,4.6mm×250mm);以甲醇-水为流动相梯度洗脱,柱温:35℃,检测波长:254nm,流速:1.0mL/min。结果:生黄芪中毛蕊异黄酮和芒柄花素的含量分别为0.2479mg/g和0.1252mg/g,炙黄芪中毛蕊异黄酮和芒柄花素的含量分别为0.2256mg/g和0.1082mg/g。结论:蜜炙法可使黄芪中毛蕊异黄酮和芒柄花素的含量降低。AIM： To compare the contents of calycosin and fermononetin between Radix Astragali and its fried product with honey. METHODS： The chromatographic column was a Waters SunfireTM C18 column（250 mm × 4. 6 mm,5 μm）. The mobile phase was the mixture of methanol and water with a linear gradient elution at a flow rate of 1 mL/min. The ultraviolet wavelength was set up 254 nm,and the column temperature was maintained at 35 ℃. RESULTS： The contents of calycosin and fermononetin in Radix Astragali were 0. 247 9 mg/g and 0. 125 2 mg/g respectively,the contents of calycosin and fermononetin in Radix Astragali fried with honey were 0. 225 6 mg/g and 0. 108 2 mg/g,respectively. CONCLUSION： The contents of calycosin and fermononetin in Radix Astragali fried with honey decreased.

关 键 词：黄芪 炙黄芪 毛蕊异黄酮 芒柄花素 

分 类 号：R283[医药卫生—中药学]