HPLC法测定决明子中大黄酚和橙黄决明素  

Determination of aurantio-obtusin and chrysophanol in Cassiae Semen by HPLC

作　　者：黄小平[1] 钟国跃[1] 张雪梅[1] 张毅[1] 

机构地区：[1]重庆市中药研究院,重庆400065

出　　处：《中国中药杂志》2010年第16期2065-2067,共3页China Journal of Chinese Materia Medica

基　　金：<中国药典>2010版一部标准研究项目(YD-073)

摘　　要：目的：修订《中国药典》2005年版决明子项下的含量测定方法。方法：采用HPLC法,以橙黄决明素和大黄酚为指标,以乙腈-0.1%磷酸溶液为流动相梯度洗脱。结果：橙黄决明素和大黄酚分别在2.3~230,2.866~286 mg.L-1与峰面积线性关系良好（r=0.999 9）,橙黄决明素和大黄酚的平均回收率分别为100.3%（RSD 3.1%）和99.5%（RSD 2.5%）。结论：修订后方法准确,专属性好,重复性好,可作为新版药典中决明子的含量测定方法。Objective： To improve the determination method of Cassiae Semen in China Pharmacopeia edition 2005.Method： Aurantio-obtusin and chrysophanol were separated on an Inertsil ODS-3 column with acetonitrile-0.1%phosphoric acid solutiona as mobile phase in gradient elution.Result： The linear ranges of aurantio-obtusin and chrysophanol were 2.3-230,2.87-286 mg·L-1,respectively.The average recover were 100.3%（RSD 3.1%）,and 99.5%（RSD 2.5%）respectively.Conclusion： This method is specific,accurate and reproducible,and can be used for the assay of Cassiae Semen in the new edition of Chinese pharmacopoeia.

关 键 词：决明子 橙黄决明素 大黄酚 HPLC 质量标准 

分 类 号：R284[医药卫生—中药学]