HPLC法测定不同产地和品种石韦中绿原酸和芒果苷的含量  

Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC

作　　者：李开通[1] 张艺轩[1] 曹阳[1] 石钺[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国中药杂志》2010年第16期2075-2078,共4页China Journal of Chinese Materia Medica

基　　金：<中国药典>2010版一部标准研究(YS-211;YS-212)

摘　　要：目的：建立石韦药材及饮片中绿原酸和芒果苷含量的HPLC测定方法。方法：色谱柱为Diamonsil（TM）钻石C18（4.6 mm×250 mm,5μm）,流动相为乙睛-0.5%磷酸水溶液梯度洗脱,检测波长320 nm。结果：绿原酸与芒果苷分别在5.2-130,1.2-18 mg.L-1线性关系良好;绿原酸的平均加样回收率97.9%,RSD 1.9%;芒果苷的平均加样回收率99.6%,RSD 2.9%。不同来源药材及饮片间绿原酸和芒果苷含量差异显著。结论：本方法对石韦药材及饮片的质量标准制定奠定了基础,也为鉴别不同品种石韦提供了参考。An HPLC method for simultaneous determination of chlorogenic acid and mangiferin in original medicinal materials and decoction pieces of Pyrrosiae Folium was developed.The assay was performed on a Didmonsil（TM） C18（4.6 mm×250 mm,5 μm）column eluted with a mobile phase consisted of acetonitrile and 0.5% phosphoric acid solution in gradient elution at a flow rate of 1.0 mL·min-1.The column temperature was set at 25 ℃.The detection wavelength was 320 nm.The results showed that The linear ranges of chlorogenic acid and mangiferin were 5.2-130 mg·L-1（r=0.999 9） and 1.2-18 μg·mL-1（r=0.999 9）,and the average recoveries（n=6） were 97.9%（RSD 1.9%） and 99.6%（RSD 2.9%）,respectively.The method was simple,reproducible and valid.It can be used for quality evaluation and control of original medicinal materials and decoction pieces of Pyrrosiae Folium.

关 键 词：石韦 绿原酸 芒果苷 高效液相色谱 质量标准 

分 类 号：R284[医药卫生—中药学]