车前草中大车前苷的定性和定量分析  

Qualitative and quantitative analysis of plantamajoside in Plantaginis Herba

作　　者：孙虔[1] 耿放[2] 程雪梅 杨莉[2] 王峥涛[1,2,3] 

机构地区：[1]中国药科大学生药学研究室,江苏南京210038 [2]上海中医药大学中药研究所中药标准化教育部重点实验室中药新资源与品质评价国家中医药管理局重点研究室,上海201210 [3]上海中药标准化研究中心,上海201210

出　　处：《中国中药杂志》2010年第16期2095-2098,共4页China Journal of Chinese Materia Medica

基　　金：国家药典委员会《中国药典》2010版一部标准研究课题(YD-017);国家“重大新药创制”重大专项(2009ZX09502-013,2009ZX09502-024);上海科委中药现代化专项基金(08DZ1970103)

摘　　要：目的：研究、建立中药车前草药材的定性定量方法。方法：以大车前苷（plantamajoside）为专属性指标成分,以硅胶G为吸附剂,醋酸乙酯-甲醇-甲酸-水（18∶3∶1.5∶1）为展开剂,紫外光灯（365 nm）下检测,建立车前草药材的薄层色谱鉴别方法。用高效液相色谱法进行含量测定,艾杰尔Promocil C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.1%甲酸水（17∶83）,流速1.0 mL.min-1,检测波长330 nm,柱温30℃。结果：车前草药材薄层色谱在紫外光灯（365 nm）下检识,大车前苷呈亮蓝色荧光斑点,斑点清晰,分离效果理想;高效液相色谱法含量测定大车前苷在0.049 9~11.966 4μg呈线性（r=0.999 9）;加样回收率为100.6%,RSD 2.7%;测得11批车前草中大车前苷的含量在0.067%~1.80%。结论：通过对11批车前草中的大车前苷的定性和定量分析,证明所建立的薄层色谱鉴别和高效液相色谱法含量测定方法灵敏,准确可靠,重复性好,为车前草的品质评价和质量标准建立提供了科学依据。Plantamajoside is one of the main bioactive compounds in Plantaginis Herba.A TLC method was developed identification of plantamajoside in 11 Plantaginis Herba samples using silica gel G as coating substance and a mixture of ethyl acetiate methanol-formic acid-water（18∶3∶1.5∶1） as a developing solvent,the established TLC condition displayed a very well separation on the chromatogram of tested Plantaginis Herba samples and the marker compound plantamajoside showed as a distinct light-blue fluorescence spot observed under UV 365 nm.Using the HPLC method,plantamajoside was separated at 30 ℃ on a Promocil C18（4.6 mm×250 mm,5 μm） column with acetonitrile-0.1%formic acid（17∶83） as the mobile phase.The detection wavelength was set at 330 nm and the flow rate was 1 mL·min-1.The calibration curve of plantamajoside displayed ideal linearity over the range of 0.049 9-11.966 4 μg（r=0.999 9）,and the average recovery of plantamajoside was 100.6%with a RSD of 2.7%.The contents of plantamajoside were in the range of 0.067%-1.80% in Plantaginis Herba.The established TLC identification and HPLC were sensitive,reliable and repeatable,which can be applied for the quality evaluation and standard criteria of Plantaginis Herba.

关 键 词：车前草 大车前苷 薄层色谱法 定性鉴别 高效液相色谱法 质量标准 

分 类 号：R284[医药卫生—中药学]