锦灯笼药材质量标准研究  

Investigation on quality standard of Franchet groundcherry

作　　者：程雪梅[1,2] 张初航 侴桂新[1,2] 王峥涛[1,2] 

机构地区：[1]上海中药标准化研究中心,上海201203 [2]上海医药大学中药研究所中药标准化教育部重点实验室,上海201203

出　　处：《中国中药杂志》2010年第16期2103-2105,共3页China Journal of Chinese Materia Medica

基　　金：<中国药典>2010版一部标准研究基金项目(YD-069);上海科委中药现代化专项(08DZ1970103)

摘　　要：目的：建立锦灯笼药材的质量标准。方法：采用薄层鉴别,以硅胶HSG为薄层板,氯仿-丙酮-甲醇（25∶1∶1）为展开剂,薄层色谱在紫外光灯（365 nm）下检识,酸浆苦味素L荧光斑点清晰,分离效果理想。高效液相色谱方法采用VenusilXBP C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈-0.2%磷酸（20∶80）为流动相,检测波长350 nm,流速1 mL.min-1对锦灯笼中的木犀草苷含量进行测定。结果：木犀草苷含量在0.50~249.40 mg.L-1呈良好的线性关系Y=55 313X＋3.164 1,r=1.000。平均回收率分别98.79%,RSD 1.1%,日内和日间精密度RSD均分别小于2%。结论：建立的定性和定量方法可用于锦灯笼药材的质量控制。Objective： To develop identification and assay methods of Franchet groundcherry.Method： TLC method was used to identify of physalin L in the sample using high performance silica gel G plate and a mixture of chloroform-acetone-methanol（25∶1∶1） as a developing solvent.In the chromatogram,physalin L showed a distinct fluorescence spot under UV 365 nm with good separation.In the HPLC method,luteoloside was separated on a Venusil XBP C18（4.6 mm×250 mm,5 μm） column with acetonitrile-0.2%phosphoric acid（20∶80） as the mobile phase with flow rate of 1 mL·min-1.The detection wavelength was set at 350 nm.Result： For the HPLC quantitation method,the calibration curve of luteoloside displayed ideal linearity over the range of 0.50-249.40 mg·L-1 with the regression equation of Y=55 313X＋3.164 1（r=1.000）.The average recovery of luteoloside was 98.79%with a RSD of 1.1%.And the intra-day and inter-day precisions were less than 2%.Conclusion： The TLC identification and HPLC determination were sensitive,reliable and repeatable and can be applied for the quality evaluation and assessment of Franchet Groundcherry Calyx.

关 键 词：酸浆苦味素L 木犀草苷 锦灯笼 薄层色谱 反相高效液相色谱 质量标准 

分 类 号：R282[医药卫生—中药学]