连翘药材新增定量标准研究  

Quantitative determination of forsythiaside in Forsythia suspensa

作　　者：夏伯侯[1] 朱晶晶[1] 王智民[1] 林丽美[1] 高慧敏[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2010年第16期2110-2112,共3页China Journal of Chinese Materia Medica

基　　金：<中国药典>2010版一部标准研究项目(YS-241);2007年中医药行业科技专项(200707009);国家自然科学基金项目(30672601);"重大新药创制"科技重大专项(2009ZX09308-003;2009ZX09301-005)

摘　　要：目的：为《中国药典》2010年版建立连翘药材中连翘酯苷A的含量测定方法。方法：采用RP-HPLC法,ZorbaxEclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈-0.4%冰醋酸溶液（15∶85）为流动相等度洗脱,检测波长330 nm,柱温25℃。结果：连翘酯苷A在0.202-1.515μg线性关系良好,r=0.999 8,平均加样回收率98.54%,RSD 1.1%;11批连翘样品中连翘酯苷质量分数在0.200%-1.681%。结论：经过交叉复核表明该方法简单、准确,可靠,重复性好。Objective： To develop a quantitative determination of Forsythia suspensa and to provide scientific basis for its quality control.Method： By HPLC,the separation was performed on a Zorebax Eclipse XDB-C18 column（4.6 mm×250 mm,5 μm） at 25 ℃ using a isocratic elution of acetonitrile-water（containing 0.4% glacial acetic acid,15∶85）.The detection wavelength was 330 nm.Result： The calibration curve showed a good linearity（r=0.999 8） within test range of 0.202-1.515 μg.And the average recovery was 98.54% with RSD 1.1%.The content range of forsythoside A in 11 batches of Forsythiae Fructus was 0.200%-1.681%.Conclusion： The developed method is simple,accurate,specific,and with good repeatability.It is suitable for quality evaluation of Forsythiae Fructus.

关 键 词：连翘 连翘酯苷A 高效液相色谱法 质量标准 

分 类 号：R284[医药卫生—中药学]