黄连合用肉桂多次灌胃对小檗碱在大鼠体内分布的影响  

Effect of Rhizoma Coptidis coadministration with Cortex Cinnamomi on tissue distribution of berberine in rats

作　　者：张瑞[1] 李凯鹏[2] 杨洁[1] 黄果[1] 杨丽娟[1] 张玉杰[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中日友好医院药剂科,北京100029

出　　处：《中国中药杂志》2010年第16期2180-2183,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672585)

摘　　要：目的:探讨黄连合用肉桂多次灌胃给予大鼠对小檗碱体内分布的影响。方法:将大鼠随机分为3组,一组作为空白,一组灌服黄连药材粉末,另一组灌服黄连与肉桂按6∶1混合的药材粉末,连续灌胃7次。采用HPLC测定给药后大鼠心、肝、脾、肺、肾等脏器中小檗碱的浓度。采用SAS统计软件进行t检验,比较黄连配伍肉桂前后各脏器中小檗碱的变化情况。结果:大鼠多次灌胃黄连后,小檗碱组织浓度升高,其在大鼠体内呈广泛分布,但主要集中在肝组织中;黄连配伍肉桂后各脏器中小檗碱浓度发生明显的改变(P<0.01),心、肝、肾中小檗碱浓度较之配伍前明显增加,而脾、肺中小檗碱浓度较配伍前明显减少。结论:黄连配伍肉桂后多次灌胃可使小檗碱在大鼠体内各脏器的分布发生明显的改变。Objective： To investigate the tissue distribution of berberine in rats after oral administration of Rhizoma Coptidis with Cortex Cinnamomi powder.Method： After oral administration of Rhizoma Coptidis powder and Rhizoma Coptidis with Cortex Cinnamomi powder（6∶1） at the dosage of 6 g·kg-1 and 6.6 g·kg-1 respectively per day for 1 week,the drug concentrations in various tissues were determined by HPLC method.The variations of berberine concentrations in tissues of two treated group of rats were compared by t test using software of SAS.Result： With repeated administration of Rhizoma Coptidis powder,berberine distributed widely in tissues of rats and the concentrations of berberine in tissues increased,yet berberine existed mainly in liver.Berberine concentrations in all organs investigated in the group of rats treated with Rhizoma Coptidis and Cortex Cinnamomi powder showed obvious difference with those of the group of rats treated with Rhizoma Coptidis powder（P0.01）.The berberine concentrations increased in heart,liver and kidney,while decreased in spleen and lung.Conclusion： Rhizoma Coptidis coadministration with Cortex Cinnamomi can obviously change the distribution of berberine in rat organs.

关 键 词：黄连 肉桂 多次灌胃 小檗碱 组织分布 

分 类 号：R285[医药卫生—中药学]