水稻白叶枯病菌北方菌株的分子鉴别和致病型分析  

PCR identification and pathotypic analysis of Xanthomonas oryzae pv. oryzae strains from North China

作　　者：阙海勇[1,2] 陈华民[1] 王继春[3] 蒋军喜[2] 吴茂森[1] 何晨阳[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 [2]江西农业大学农学院,南昌330045 [3]吉林省农业科学院植物保护研究所,公主岭136100

出　　处：《植物病理学报》2010年第4期351-356,共6页Acta Phytopathologica Sinica

基　　金：国家农业行业科研专项经费项目(3-23);植物病虫害生物学国家重点实验室开放基金课题(SKL2009OP03);国家科技支撑计划项目(2006BAD08A04)

摘　　要：为了阐明水稻白叶枯病菌（Xanthomonas oryzaepv.oryzae,Xoo）北方菌株的毒性组成,对2008年和2009年从辽宁、吉林、河北、山东稻区采集的103个菌株进行了PCR分子鉴定,并利用一套水稻抗白叶枯病近等基因系作为鉴别品种,进行了致病性测定。结果表明,用3种不同引物对测定菌株基因组进行PCR扩增,均可以鉴别出与Xoo代表菌株完全一致、而与水稻条斑病菌明显不同的特异性扩增片段带型。6个水稻鉴别品种对测定菌株的感病反应明显不同,抗病性强弱依次为IRBB5〉IRBB13〉IRBB3〉IRBB14〉IRBB2〉IRBB24。103个菌株对6个水稻鉴别品种表现出毒力多样性,92个菌株（89.32%）表现与9个标准小种（R1~R9）代表菌株相同的致病型,其中36个R5和R8菌株为优势菌株,9个菌株为对6个水稻品种都致病的R9强毒力菌株;此外,新发现了其余11个菌株（10.68%）具有7种新的致病型（R10~R16）。To demonstrate the field prevalence and virulence structure of Xanthomonas oryzae pv.oryzae（Xoo）strains in the north rice-growing regions of China,PCR analysis using 3 pairs specific primers of the genomic DNA of 103 bacterial strains isolated from diseased leaves of rice from Liaoning,Jilin,Hebei and Shandong Provinces was conducted in 2008 and 2009,respectively,and the bacterial pathogenicity on 6 near-isogenic lines of rice（IRBB2,IRBB3,IRBB5,IRBB13,IRBB14 and IR24）containing single Xa resistance gene,which as a set of differential varieties was also tested.The results showed that genomic DNA fragment patterns amplified by PCR of all tested strains were the same as those of the representatives of 9 pathotypes/races（R1-R9）of Xoo,but not Xanthomonas oryzae pv.oryzicola（Xoc）.The rice differential varieties showed different resistant and susceptible reactions to Xoo strains,and the sequence of resistance was IRBB5IRBB13IRBB3IRBB14IRBB2IR24.Moreover,the diversity in virulence of Xoo strains on 6 rice near-isogenic lines was observed.Ninety-two strains（89.32%）displayed the same pathotypes of R1-R9,among which 36 strains of R5 and R8 were dominant in population and 9 strains of R9 were virulent on all rice varieties used.Remarkably,7 new pathotypes（R10-R16）of 11 strains were identified as well.

关 键 词：水稻白叶枯病菌 PCR 近等基因系 鉴别品种 致病型 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]