外源茉莉酸甲酯诱导水稻抗瘟性相关防御酶和内源水杨酸的变化  

Resistance-related defense enzymes and endogenous salicylic acid induced by exo-genous methyl jasmonate in rice against blast disease

作　　者：张智慧[1,2] 聂燕芳[3] 何磊[1,2] 李云锋[1,2] 王振中[1,2] 

机构地区：[1]华南农业大学植物病理生理学研究室,广州510642 [2]教育部生物防治工程研究中心,广州510642 [3]华南农业大学制药工程系,广州510642

出　　处：《植物病理学报》2010年第4期395-403,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30971887;30600399);广东省自然科学基金资助项目(07006683;05300351)

摘　　要：用外源茉莉酸甲酯(MeJA)处理抗稻瘟病近等基因系水稻CO39和C101LAC,显著减轻了稻瘟病的发生,而对稻瘟病菌孢子萌发及菌丝生长均无明显抑制作用,证实MeJA处理后稻瘟病病情指数的下降是由于MeJA提高了水稻幼苗的抗瘟性。对水稻抗瘟性重要防御酶的活性测定结果表明,苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、过氧化氢酶(CAT)和脂氧合酶(LOX)活性均在MeJA处理早期上升,与亲和性互作水稻相比,高度非亲和性互作水稻中的诱导活性增加明显,且速度快。对病程相关蛋白β-1,3-葡聚糖酶和几丁质酶的活性测定结果表明,高度非亲和性互作水稻PR蛋白活性的高峰期出现和强度也明显要早且高于亲和性互作水稻。对内源水杨酸(SA)的测定结果表明,不同亲和性互作水稻的SA含量均没有明显的变化,表明MeJA诱导的水稻信号通路可能与SA信号通路无关。Methyl jasmonate（MeJA）is important signaling hormones in plants,known to play a role in developmental processes and in induced defense responses.Exogenous MeJA significantly increased disease resistance of rice against rice blast,but no inhibitory effect was detected on spore germination and mycelium growth of Magnaporthe grisea in vitro.Therefore,the reduction of disease severity in MeJA-treated rice plants was due to MeJA-induced resistance to blast.After treatment with MeJA,changes in LOX,PAL,POD and CAT activities increased significantly compared with those in contro1.Compared with the compatible rice line,CO39,the activities of four enzymes increased more remarkably and rapidly in the leaves of the incompatible rice line C101LAC.PR proteins were possessed defining characteristic of systemic acquired resistance（SAR）in plants.The results showed that changes in two pathogenesis-related proteins（PR proteins）,chitinase and β-1,3-glucanase,were also increased more remarkably and rapidly in the leaves of the incompatible rice line than that of compatible rice line.Our results also showed that endogenous SA had little change either in the leaves of incompatible rice line or compatible line.The results suggested that defense-related enzymes and SAR in rice leaves induced by MeJA might play an important role in resistance to rice blast.

关 键 词：茉莉酸甲酯 水稻 稻瘟病 防御酶 内源水杨酸 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]