大麦条纹花叶病毒中国株三基因盒(TGB)序列对比分析  

Comparing and analyzing the tripe gene block sequence of Barley stripe mosaic virus China strain

作　　者：孙现超[1] 赵文军[2] 青玲[1] 杨水英[1] 

机构地区：[1]西南大学植物保护学院,重庆400716 [2]中国检验检疫科学研究院动植物检疫研究所,北京100029

出　　处：《植物病理学报》2010年第4期433-437,共5页Acta Phytopathologica Sinica

基　　金：中国博士后科学基金(20090450798);重庆市自然科学基金(CSTC.2007BB1349)

摘　　要：The tripe gene block(TGB)genes of Barley stripe mosaic virus China strain(BSMV-CH)were amplified from cDNA of BSMV-CH RNAβ(GenBank accession No:AY789694)by PCR with special primer pairs,and cloned into pMD18-T vector for sequencing.Analysis of the sequences showed that the full length of BSMV-CH TGB1,TGB2 and TGB3 were 1 539,396 and 468 bp,with deduced 512,131 and 155 amino acids,respectively.BSMV-CH TGB1 shared 94.1%-95.4% nucleotide identities and 91.0%-94.5% amino acid identities with that of other BSMV strains,BSMV-CH TGB2 shared 96.5%-97.2% nucleotide identities and 98.5%-99.2% amino acid identities with that of other BSMV strains,and BSMV-CH TGB3 shared 95.7%-96.6% nucleotide identities and 94.2%-96.8% amino acid identities with that of other BSMV strains.Phylogenetic tree based on the amino acid of Hordeiviruses TGB genes showed that BSMV-CH was relatively closed to CV17 in genetic relationship.Therefore,BSMV-CH was deduced to be a recombined strain of CV17 and CV42.The tripe gene block（TGB）genes of Barley stripe mosaic virus China strain（BSMV-CH）were amplified from cDNA of BSMV-CH RNAβ（GenBank accession No：AY789694）by PCR with special primer pairs,and cloned into pMD18-T vector for sequencing.Analysis of the sequences showed that the full length of BSMV-CH TGB1,TGB2 and TGB3 were 1 539,396 and 468 bp,with deduced 512,131 and 155 amino acids,respectively.BSMV-CH TGB1 shared 94.1%-95.4% nucleotide identities and 91.0%-94.5% amino acid identities with that of other BSMV strains,BSMV-CH TGB2 shared 96.5%-97.2% nucleotide identities and 98.5%-99.2% amino acid identities with that of other BSMV strains,and BSMV-CH TGB3 shared 95.7%-96.6% nucleotide identities and 94.2%-96.8% amino acid identities with that of other BSMV strains.Phylogenetic tree based on the amino acid of Hordeiviruses TGB genes showed that BSMV-CH was relatively closed to CV17 in genetic relationship.Therefore,BSMV-CH was deduced to be a recombined strain of CV17 and CV42.

关 键 词：烟草花叶病毒 序列对 基因盒 中国株 运动蛋白 条纹 大麦 马铃薯X病毒 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]