围绝经期抑郁症模型大鼠空间学习记忆和海马超微结构的变化  

Changes of spacial learning and memory abilities and ultrastructure of hippocampus in perimenopausal depression model rats

作　　者：肖爱娇[1] 熊浩仲[2] 周军[3] 

机构地区：[1]江西中医学院生理学教研室,南昌330004 [2]江西中医学院基础医学院,南昌330004 [3]江西中医学院生物化学教研室,南昌330004

出　　处：《中华行为医学与脑科学杂志》2010年第8期692-694,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：江西省卫生厅中医药科研计划资助项目（2009A006）

摘　　要：目的 观察围绝经期抑郁症模型大鼠空间学习记忆及海马形态结构的变化.方法 雌性SD大鼠36只,分为正常组、应激组、去势组和模型组.正常组不给予任何处理,应激组给予孤养加2l d慢性不可预见性的温和应激（CUMS）,去势组除卵巢切除术外不给予任何应激,模型组在卵巢切除术的基础上,给予孤养加2l d CUMS.采用Morris水迷宫测试大鼠的学习记忆力;光镜以及透射电镜观察大鼠海马组织结构.结果 2ld应激后,模型组大鼠前3d的逃避潜伏期分别为[（44.08±18.29）s;（38.80±19.07）s;（22.74±14.13）s],长于去势组大鼠[（43.68±10.37）s;（20.28±17.75）s;（21.22±11.91）s]和正常组大鼠[（34.50±11.08）s;（21.42±14.32）s;（13.94±9.76）s],且模型组大鼠搜索平台的策略多为边缘型和随机型.HE染色显示模型组大鼠海马锥体细胞层变薄,细胞间隙增大,排列紊乱,疏松,细胞结构不完整,甚至有大量细胞缺失;透射电镜下,模型组海马神经元细胞核呈现皱缩改变,核膜凹凸不整模糊不清,并有局部断裂;胞质内线粒体肿胀变性,嵴消失.结论 围绝经期抑郁症大鼠模型存在学习记忆力下降,这可能与应激造成的海马形态结构改变有关.Objective To observe changes of spacial learning and memory and the ultrastructure of hippocampus in perimenopausal depression model rats. Methods Thirty-six female SD rats were randomly divided into four groups（9 in each）. Normal group wasn＇t given any stress, and stressed group was given isolation-housing in combination with chronic unexpected mild stress （CUMS） for 21 days. Ovariectomized group was given ovary resecting, and model group was made by resecting the ovaries of female rats, then giving isolation-housing in combination with 21 days of CUMS. Morris water maze test were used to test rats＇ behaviors. Light microscope and transmission electron microscope were used to observe the tissular structure of hippocampus. Results After ovaries resecting and 21 days of CUMS,the escape periods of rats in model group during the first three days （ （44.08 ± 18.29）s; （ 38.80 ± 19.07 ） s; （ 22.74 ± 14.13 ） s） were longer than ovariectomizd group （ （43.68 ± 10. 37 ） s; （ 20.28 ±17.75）s;（21.22 ± 11.91 ）s] and normal group（ （34.50 ± 11.08）s;（21.42 ± 14.32） s;（ 13.94 ±9.76） s）. HE staining showed that hippocampal pyramidal cell layer became thinner in rat model of depression,the gap between cells increased,and the cells arrayed in disorder,or loosely,even a large number of cells were lost. Transmission electron microscope showed that in rats of model group, hippocampal neurons were less, nuclear collapsed, the whole nuclear membrane was fuzzy and there were some local fractures in the nuclear membrane. Mitochondria within the cytoplasm were swelling and their ridges disappeared. Conclusion The decline of spacial learning and memory abilities in perimenopausal depression model rats might result from pathological changes in hippocampus.

关 键 词：慢性不可预见性的温和应激 围绝经期抑郁症 海马 透射电镜 

分 类 号：R285.5[医药卫生—中药学]