电晕电场对细枝岩黄芪种子愈伤组织诱导与分化的影响  

EFFECT OF FIELDS RADIATION ON CALLUS INDUCTIVITY AND DIFFERENTIATION OF Hedysarum scoparium Fisch. et Mey.

作　　者：那日[1,2] 杨生[3] 邢万金[4] 杨体强[1,2] 

机构地区：[1]内蒙古自治区离子束生物工程重点实验室,内蒙古呼和浩特010021 [2]内蒙古大学物理科学与技术学院,内蒙古呼和浩特010021 [3]中国农业科学院草原研究所,内蒙古呼和浩特010010 [4]内蒙古大学生命科学学院,内蒙古呼和浩特010021

出　　处：《核农学报》2010年第4期748-751,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金委员会项目(50767002);内蒙古自治区自然科学基金重大项目(200711020101)

摘　　要：研究了不同电场剂量处理细枝岩黄芪种子对其愈伤组织诱导率和分化率的影响。结果表明,电场处理可以明显提高愈伤组织诱导率和分化率。电场强度为7kV/m,处理时间为100min,诱导培养基为MS+2,4-D 2.0mg/L+6-BA 1.0mg/L时出愈率可达91.0%,且生长情况较好,褐变程度轻。在MS+2,4-D 2.0mg/L+6-BA1.0mg/L诱导培养基上继代培养后的各愈伤组织分别转移到分化培养基上进行分化培养,当电场强度为7kV/m,时间为100min时分化率最高,达到78.3%。The seeds of Hedysarum scoparium Fisch.et Mey.were treated by electrical field at different doses,the effect on the callus inductivity and differentiation rate were studied.The results showed that electrical field treatment could increase the callus inductivity and differentiation rate,when the field strength was 7kV /m,treating time was 100min and the inducing media was MS ＋ 2,4-D 2.0mg/L ＋ 6-BA 1.0mg/L,the callus inductivity was 91.0% with low browning rate.The callus differentiation rate 78.3% on the medium with MS ＋ 6-BA 2.0mg/L ＋ NAA 0.2mg /L with field strength of 7kV/m and time of 100min was the highest.

关 键 词：细枝岩黄芪 电晕电场 愈伤组织诱导 分化 

分 类 号：S567.239[农业科学—中草药栽培]