金标孕酮单克隆抗体复合物的制备及鉴定  

Research on Development of Gold-monoclonal Antibody of Progesterone under the Optimized Conditions

作　　者：许欣[1] 孟立[1] 王子玉[1] 王锋[1] 

机构地区：[1]南京农业大学动物胚胎工程技术中心,南京210095

出　　处：《中国畜牧兽医》2010年第8期55-58,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划课题(2008BADB2B09;2008BADB2B04);江苏省科技厅高技术研究项目(BG2007324)

摘　　要：为了获得高质量的金标孕酮单抗,通过采用柠檬酸三钠还原法,制备出符合要求的30nm胶体金溶液,在此基础上摸索胶体金标记孕酮单克隆抗体的最佳条件,并对其进行质量鉴定。结果发现,用柠檬酸三钠还原法制备的30nm胶体金溶液呈深红色,透射电镜观察,胶体金颗粒大小基本一致;胶体金标记孕酮单克隆抗体的最低稳定浓度为16μg/mL,最适稳定浓度为19.2μg/mL,最适标记pH为8.5。制备的金标抗体复合物经电镜观察,周围有一层明显的低电子密度晕圈,且其D520nm值在0.2～0.4之间。In order to get high quality gold-monoclonal antibody of progesterone, the colloidal gold particles of 30 nm was prepared by the tri-sodium citrate reduction method, and then identified by two different ways. The key experiment conditions to develop gold-monoclonal antibody of progesterone were optimized. The spheroidal colloid gold particles of 30 nm was shown in color of bright red, and the equal particle ultrastructure was observed through transmission electron microscope（TEM）; the minimal stable concentration （MSC） of gold-monoclonal antibody was 16 μg/mL, and the suitable stable concentration （SSC） was 19.2 μg/mL. The pH 8.5 was appropriate. Obvious low electron density halo was obversed around the particle of gold-monoclonal antibody complex, and the D520 nm was in the range of 0.2 to 0.4, thereby preparing for further research on the gold immunochromatography test strip of rapid diagnosis of pregnancy.

关 键 词：胶体金 孕酮单克隆抗体 金标单抗 

分 类 号：S852.43[农业科学—基础兽医学]