乙型脑炎病毒减毒SA14-14-2-B3株基因组全序列分析  

Complete genome sequence analysis of of Japanese encephalitis virus attenuated SA14-14-2-B3 vaccine strain

作　　者：张学涛[1] 赵明秋[1] 琚春梅[1] 赵启祖[2] 康艳梅[1] 沈海燕[1] 陈金顶[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]中国兽医药品监察所,北京100081

出　　处：《中国预防兽医学报》2010年第8期632-634,654,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：农业公益性行业科研专项(200803015);广东省自然科学基金创新团队项目(5200638);教育部"长江学者和创新团队发展计划"创新团队项目(TRT0723);广东省科技计划项目(2008B020600003)

摘　　要：为进一步了解乙型脑炎病毒(JEV)减毒疫苗株基因组变异和分子遗传特性,本研究根据已发表的JEV基因组序列,设计合成了8对引物,应用RT-PCR对SA14-14-2-B3株基因组进行了克隆和序列测定,获得了该株病毒的全基因组序列(10977nt)并推导出其编码的氨基酸序列(3432aa)。序列分析表明:SA14-14-2-B3株与疫苗株SA14-14-2和SA(A)及野毒株SA14的核苷酸和氨基酸相似性较高,分别为99.8%、99.9%、99.4%和99.7%、99.8%、99.1%,与猪源分离株HEN0701和KV1899的核苷酸与氨基酸相似性较低,分别为88.5%、88.5%和97.6%、96.6%,比较显示在SA14-14-2-B3株基因组第10700nt处有一碱基"G"的插入。以全基因组为基础,对SA14-14-2-B3株进行遗传进化关系分析,结果表明:SA14-14-2-B3株与SA14源病毒株SA14、SA14-14-2、SA(A)、SA(V)及分离株Beijing-1、p3、WHe和HW的遗传关系较近,与XJP613株和HEN0701株的遗传关系较远。以E基因为基础,对SA14-14-2-B3株进行遗传进化关系分析,结果表明SA14-14-2-B3株属于JEV基因Ⅲ型。The complete genome sequence of Japanese encephalitis virus （JEV） SA14-14-2-B3 strain was amplified by RT-PCR using eight pairs of primers designed according to the published sequence of JEV. The genome consisted of 10 977 nt, encoding a poly-protein of 3 432 amino acids （aa） residues. Sequence comparison showed that it had high level identities with the vaccine strain SA14-14-2, SA （A） and wildtype strain SA14, with 99.8 %, 99.9 % and 99.4 % homologies at nucleotide level and 99.7 %, 99.8 % and 99.1 % homologies at amino acid level, respectively. The sequence identities with HEN0701 and KV1899 of porcine origin was relatively lower, with 88.5 % similarities in nucleotide and 96.6 %, 97.6 % in amino acids. There was a G-base insertion at the site of 10 700 nt in SA14-14-2-B3 strain. Phylogenetic analysis showed that the SA14-14-2-B3 strain had a closest genetic relationship with SA14 virus strains SA14, SA14-14-2, SA （A）, SA （V）, and Beijing-1, p3, WHe, HW isolates, but a low genetic similarity with HEN0701 and KV1899 isolates. Phylogenetic relationship based on the E gene indicated that the SA14-14-2-B3 strain belonged to JEV genotype Ⅲ.

关 键 词：乙型脑炎病毒 SA14-14-2-B3株 基因组 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]