盐酸奥芬米洛对大鼠实验性自身免疫性脑脊髓炎的预防作用及其机制  

Preventive effect of oxfenmino on experimental autoimmune encephalomyelitis in rats and its mechanism

作　　者：寿旗扬[1] 屠珏[1] 贾临超[1] 方明笋[1] 蔡月琴[1] 陈民利[1] 

机构地区：[1]浙江中医药大学动物实验研究中心,浙江杭州310053

出　　处：《中国新药与临床杂志》2010年第7期506-510,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：浙江省医药卫生科学研究基金(2007B152);浙江省卫生高层次创新人才培养工程项目资助

摘　　要：目的探讨奥芬米洛对实验性自身免疫性脑脊髓炎(EAE)大鼠发病的预防作用及其机制。方法 40只大鼠随机分为正常对照组、模型对照组、环孢素(10 mg·k^(-1))组和奥芬米洛(1.0 mg·kg^(-1))组,每组10只。除正常对照组大鼠外,其余各组大鼠双后脚掌皮下注射豚鼠全脊髓匀浆-完全福氏佐剂(GPSCH-CFA)0.4 mL进行免疫,同时奥芬米洛组和环孢素组经口灌胃给予相应的药物,每日1次,连续给药18 d。观察各组大鼠体重、神经功能评分、MBP抗体、Th1细胞因子(IFN-γ、TNF-α和IL-2)和Bcl-2/Bax蛋白表达的变化。结果奥芬米洛组EAE大鼠无临床症状发生;与模型对照组比较,奥芬米洛组大鼠血清中MBP抗体、IFN-γ、TNF-α和IL-2水平均明显降低(P<0.05或P<0.01),大脑皮质和下丘脑Bcl-2蛋白表达均明显升高(P<0.05或P<0.01),大脑皮质Bax蛋白表达明显降低(P<0.05)。结论奥芬米洛能抑制EAE大鼠的发病,其作用机制可能与抑制Th1细胞活性有关。AIM To investigate the preventive effect of oxfenmino on experimental autoimmune encephalomyelitis （EAE） in rats and its mechanism. METHODS Forty rats were randomly divided into normal control group, model control group, ciclosporin group, and oxfenmino group, 10 for each group. Except the normal control group, hind paw of rats in the other three groups were injected GPSCH-CFA 0.4 mL for immunization, meanwhile oxfenmino group and ciclosporin group were orally administered oxfenmino （1.0 mg·kg^-1） and ciclosporin （10 mg·kg^-1） everyday, continued for eighteen days. The weight, clinical scores, and MBP antibody were tested and the changes of Th1 cytokines （IFN-γ, TNF-α, and IL-2） and Bcl-2/Bax expression in brain were observed. RESULTS The EAE rats administrated with oxfenmino 1.0 mg·kg^-1 exhibited no morbidity and nervous symptom in the oxfenmino group; compared with the model control group, the levels of MBP antibody, IFN-γ, TNF-α, and IL-2 in serum decreased remarkably （P 〈 0.05 or P 〈 0.01）, the Bcl-2 expression in pallium and hypothalamus increased （P 〈 0.05 or P 〈 0.01）, and the Bax expression in pallium depressed （P 〈 0.05） in the oxfenmino group. CONCLUSION Oxfenmino has a significiant effect in preventing EAE in rats, and the mechanism may be related to inhibiting Th1 cell activity.

关 键 词：奥芬米洛 脑脊髓炎 自身免疫性 实验性 大鼠 TH1细胞因子 BCL-2/BAX蛋白表达 

分 类 号：R961[医药卫生—药理学] R593[医药卫生—药学]