辛伐他汀对去卵巢大鼠股骨骨折愈合的影响  

Effects of simvastatin on femur fracture healing in ovariectomized rats

作　　者：田发明[1,2] 张柳[1,2] 穆树林[2] 李震[2] 李强[2] 程潭[1] 

机构地区：[1]河北医科大学,河北石家庄050017 [2]华北煤炭医学院附属医院骨科,河北唐山063000

出　　处：《中国新药与临床杂志》2010年第7期511-515,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：河北省自然科学基金资助项目(C2006000580)

摘　　要：目的研究辛伐他汀对去卵巢大鼠股骨干骨折愈合的影响。方法 40只雌性、12周龄SD大鼠随机分成4组,每组10只。A组仅暴露腹腔,不切除卵巢,其余3组行卵巢切除,C、D组同时制备股骨干中段横行骨折模型。术后D组大鼠给予辛伐他汀5 mg·kg^(-1)灌胃,隔日1次,其余各组给予生理盐水。于术后5 wk处死大鼠,测量右股骨整体骨密度(tBMD)、远端骨密度(dBMD)和中段骨密度(mBMD),观察C、D组大鼠骨折愈合情况以及骨形成蛋白2(BMP-2)、血管内皮生长因子(VEGF)的表达。结果 D组较C组骨痂量相对较多,骨折线模糊;B组tBMD和dBMD明显低于A组,D组mBMD明显高于C组,差异均有显著意义(P<0.05);D组tBMD和dBMD较C组有增高趋势,但无显著差异(P>0.05)。D组与C组比较,板层骨形成增加,骨痂组织BMP-2、VEGF的表达均增高(P<0.05)。结论辛伐他汀可促进去卵巢大鼠股骨骨折愈合,其机制可能与上调骨痂组织BMP-2、VEGF的表达,加快编织骨向板层骨的转化相关。AIM To evaluate the effects of simvastatin on femur fracture healing in bilateral ovariectomized rats. METHODS Forty 12-week-old female Sprague-Dawley rats were randomized into four groups of ten animals each and were either subjected to sham （group A） or bilateral ovariectomy operation （group B, C and D）. Femur fracture was carried in group C and D. After the operation, rats were administered with simvastatin 5 mg·kg^-1 （group D） or equivalent normal saline （Group A, B and C） once per two days for 5 weeks. All rats were sacrificed 5 weeks after operation. The bone mineral density of distal （dBMD） , middle （mBMD） and total （tBMD） area of the right femur was determined by dual-energy X-ray absorptiometry. In group C and D, fracture healing was observed and the expression of bone morphogenetic protein 2 （BMP-2） and vascular endothelial growth factor （VEGF） was evaluated using immunohistochemistry. RESULTS The tBMD and the dBMD of group B were far less than those of group A, the mBMD of group D was significantly higher than that of group C （P 〈 0.05）. The tBMD and the dBMD of group D were higher than those of group C, but there were no significant difference （P 〉 0.05）. Compared with group C, significantly higher expression levels of BMP-2 and VEGF, as well as more lamellar bone were observed in group D （P 〈 0.05）. CONCLUSION Simvastatin can accelerate the fracture healing in ovariectomied rats, which might rely on increasing the expression of BMP-2 and VEGF and thereby speeding the transformation of woven bone into lamellar bone.

关 键 词：骨质疏松 骨折愈合 辛伐他汀 骨形态发生蛋白质类 血管内皮生长因子类 

分 类 号：R68[医药卫生—骨科学] R972.6[医药卫生—外科学]