干旱胁迫对华北绣线菊和金山绣线菊光合能力的影响  

Effects of drought stress on photosynthesis capability of Spiraea fritschiana and Spiraea bunmalba 'Goldmound'.

作　　者：刘慧民[1,2] 车艳双[1] 车代弟[1] 闫永庆[1] 吴凤芝[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]东北林业大学林学院博士后流动站,哈尔滨150040

出　　处：《应用生态学报》2010年第8期2004-2009,共6页Chinese Journal of Applied Ecology

基　　金：东北农业大学博士启动项目(2009);东北农业大学创新团队项目(CXZ004-3)资助;黑龙江省度博士后基金项目(2009);黑龙江省教育厅项目(2010)

摘　　要：分别采取轻度、中度、重度干旱胁迫和复水处理,研究华北绣线菊和金山绣线菊的光合能力动态变化;利用二维双向电泳与质谱鉴定等技术,分析鉴定干旱胁迫前后2种绣线菊蛋白质的差异表达,以及引起其光合能力改变的生理机制.结果表明:干旱胁迫处理显著影响了2种绣线菊的光合能力,最大光合速率、光补偿点和光饱和点逐渐下降,其干旱胁迫反应为渐进效应.轻度和中度干旱胁迫后,2种绣线菊的恢复能力较强;而重度干旱胁迫后的恢复能力较弱.经干旱胁迫诱导后,抗旱能力弱的金山绣线菊有6处蛋白点消失、11处新增蛋白点、13处蛋白点上调表达、4处蛋白点下调表达,均为低分子量酸性蛋白;其中由干旱诱导表达的3种差异蛋白分别为放氧增强蛋白因子1、2和1,5-二磷酸核酮糖羧化酶/加氧酶大亚基的降解片断.绣线菊抗旱能力的差异与干旱胁迫期间光合能力的变化有关.In this paper,Spiraea fritschiana and Spiraea bunmalba ＇Goldmound＇ were treated with mild,moderate,and severe drought to study the dynamic changes of their photosynthesis capability,and two-dimensional electrophoresis and mass spectrometry were adopted to analyze and identify the differences in the protein expression of the two species before and after the treatments,and the physiological mechanisms inducing the changes of the photosynthesis capability.Drought treatments had significant effects on the photosynthesis capability of the two species.Under drought stress,the maximum photosynthetic rate,light compensation point,and light saturation point decreased gradually,suggesting that the responses of the two species to drought stress were progressive.The two species presented stronger recovery capability after the mild and moderate stresses,but weaker recovery capability after severe stress.After the inducement of drought stress,the weaker drought-resistant S.bunmalba ＇Goldmound＇ had six protein spots lost,eleven new protein spots appeared,thirteen protein spots up-regulation expression,and four protein spots down-regulation expression.All of the proteins were low molecular weight acidic proteins,of which,there were three kinds of different proteins that had been induced expression by drought and were the oxygen-enhanced protein factor 1 and 2 and the degradation fragments of large subunit 1,5-ribulose bisphosphate carboxylase/oxygenase.The drought-resistant difference of the two Spiraea species was related to the changes of their photosynthesis capability during drought stress.

关 键 词：干旱胁迫 绣线菊 光合能力 差异蛋白质 

分 类 号：Q945.78[生物学—植物学]