嫁接对铜胁迫下黄瓜幼苗根系多胺代谢的影响  

Effects of grafting on root polyamine metabolism of cucumber seedlings under copper stress.

作　　者：张自坤[1,2,3] 刘世琦[1,2,3] 刘素慧[1] 张宇[1] 陈昆[1] 黄治军[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]作物生物学国家重点实验室,山东泰安271018 [3]农业部园艺作物生物学重点实验室,山东泰安271018

出　　处：《应用生态学报》2010年第8期2051-2056,共6页Chinese Journal of Applied Ecology

基　　金：山东农业大学青年科技创新基金项目(23653);公益性行业(农业)科研专项(200903018)资助

摘　　要：采用营养液栽培法,研究了嫁接(以黑籽南瓜为砧木)对铜胁迫下黄瓜幼苗根系活力及多胺代谢的影响.结果表明:铜胁迫下黄瓜幼苗根系活力下降,电解质渗漏率升高,而嫁接苗的变化幅度显著小于黄瓜自根苗;铜胁迫下黄瓜嫁接植株根系中除游离态腐胺(Put)含量显著低于自根苗外,结合态和束缚态Put、3种形态亚精胺(Spd)和精胺(Spm)含量均显著高于自根苗,嫁接苗根系中游离态Put含量及腐胺/多胺(Put/PAs)显著低于自根苗;铜胁迫下,嫁接苗根系精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)和S-腺苷蛋氨酸脱羧酶(SAMDC)活性高于自根苗,而二胺氧化酶(DAO)和多胺氧化酶(PAO)活性显著低于自根苗.表明嫁接黄瓜幼苗根系PAs的合成增加,降解减少,使PAs含量维持在较高水平,从而提高了黄瓜幼苗抗铜胁迫能力.By the method of hydroponic culture,and taking Cucurbita ficifolia B.as rootstock,this paper studied the effects of grafting on the root polyamine metabolism of cucumber seedlings under copper stress.The results showed that under copper stress,the root activities of cucumber seedlings were inhibited,and electrolyte leakage increased,with these changes being significantly lower for grafted than for ungrafted cucumber seedlings.In addition,the contents of free spermidine and spermine,and of conjugated and bound polyamines were significantly higher in grafted than in ungrafted seedling roots,while the free putrescine content and the ratio of free putrescine to polyamines were on the contrary.Comparing with those in ungrafted cucumber seedlings,the root arginine decarboxylase（ADC）,ornithine decarboxylase（ODC）,and S-adenosylmethionine decarboxylase（SAMDC） activities in grafted cucumber seedlings were higher,while the diamine oxidase and polyamine oxidase activities were significantly lower.All of these indicated that under copper stress,the synthesis of polyamine in grafted seedling roots was increased,while the degradation of polyamine was decreased,resulting in a higher accumulation of polyamine in the roots,and the increase of the tolerance of cucumber seedlings to copper stress.

关 键 词：嫁接 铜胁迫 黑籽南瓜 多胺代谢 

分 类 号：S642.2[农业科学—蔬菜学]