苦地胆内酯类化合物的制备及其体外抑菌效果研究  

Preparation of lactonic extract from Elephantopus scaber and its antibacterial effects in vitro

作　　者：刘勇[1] 聂芳红[2] 林红英[1] 陈进军[1] 马驿[1] 巨向红[1] 

机构地区：[1]广东海洋大学动物医学系,广东湛江524088 [2]广东海洋大学食品科技学院,广东湛江524088

出　　处：《中国兽医科学》2010年第1期107-110,共4页Chinese Veterinary Science

基　　金：湛江科技攻关计划项目(湛科[2006]94号);广东省科技攻关计划重点引导项目(2004B20201007)

摘　　要：为探讨中药苦地胆有效成分苦地胆内酯的抗菌效果,用浓度为800mL/L的乙醇溶液进行热回流浸提,制备苦地胆内酯的粗提物,经硅胶柱层析分离、纯化,再结晶,制得苦地胆内酯类化合物,用薄层层析法进行苦地胆内酯检测与鉴定,并进行了苦地胆内酯类化合物的体外抑菌试验。结果显示,当浓度为2.0mg/mL时,苦地胆内酯类化合物对巴氏杆菌、蜡状芽孢杆菌具有明显的抑制作用(P<0.05),对金黄色葡萄球菌、肠炎沙门氏菌和鸡大肠杆菌呈现极明显的抑制作用(P<0.01);浓度分别为50、10mg/mL时,苦地胆内酯类化合物对5种供试菌均有极明显的抑制作用(P<0.01)。苦地胆内酯类化合物对供试金黄色葡萄球菌、肠炎沙门氏菌、巴氏杆菌、鸡大肠杆菌、蜡状芽孢杆菌的最小抑菌浓度分别为0.313、0.313、0.156、0.625mg/mL和1.25mg/mL;对蜡状芽孢杆菌的最小杀菌浓度为2.5mg/mL,对其他4种供试菌的最小杀菌浓度均为0.625mg/mL。表明,苦地胆内酯类化合物具有很好的体外抗菌效果。To investigate the antibacterial effects of lactones from Elephantopus scaber,a well-known Chinese herbal medicine, ku-di-dan, the crude extracts from E. scaber were prepared with 800 mL/L ethanol and followed by purification with silica gel column chromatography and crystallization. The purified extract of lactones from E. scaber （PELES） was checked and identified by thin layer chromatography and its anti-bacterial effect in vitro was observed. 2.0 mg/mL of PELES inhibited the growth of Pasteurella multocida （PM） and Bacillus cereus（BC）（P〈0.05）,and significantly inhibited the growth of Staphylococcus aureus （SAP ,Salmonella enteritidis （SE） ,and Escherichia coli in chicken（CC） （P〈0.01）, respectively. PELES at 5 mg/mL and 10 mg/mL significantly inhibited（P〈0.01） the growth of all the five strains of bacteria（P〈 0.01） and the minimum inhibitory concentration（MIC） of PELES was 0. 313 mg/mL against SA and SE and 0. 156 mg/mL against PM,0. 625 mg/mL against CC,and 1.25 mg/mL against BC. The minimum bac-tericidal concentration（MBC） of PELES against BC was 2.5 mg/mL while the MBC against the other four bacteria was 0. 625 mg/mL. The result showed that PELES from ku-di-dan had significant antibacterial effects.

关 键 词：苦地胆内酯 提取纯化 薄层层析 体外抑菌作用 

分 类 号：S853.74[农业科学—临床兽医学] R284.2[农业科学—兽医学]