烹调油烟多环芳烃暴露与职业接触人群DNA氧化性损伤  

Association of cooking oil fumes exposure and oxidative DNA damage among occupational exposed populations

作　　者：柯跃斌[1,2] 徐新云[2] 袁建辉[2] 房师松[2] 刘益民[2] 邬堂春[1] 

机构地区：[1]华中科技大学同济医学院劳动卫生与环境卫生学系,武汉430030 [2]深圳市疾病预防控制中心毒理学研究室

出　　处：《中华劳动卫生职业病杂志》2010年第8期574-578,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30872149/C1504）;广东省自然科学基金项目（8451802003000336）

摘　　要：目的 通过调查不同组别厨师和非暴露组尿中8-羟基脱氧鸟苷（8-OHdG）的排泄量,研究8-OHdG和1-羟基芘（1-OHP）之间的关系.方法 采集不同组别厨师组（n=86）和非暴露对照组（n=36）的尿液样本,观察对象均为年龄22～28岁男性,并有相似的吸烟习惯.在采样之前24 h内,以问卷调查的形式对研究对象的身体健康状况、职业史、吸烟习惯和酒精消费量进行评估.冷藏尿液样本,随后通过高效液相色谱法分析8-OHdG和1-OHP水平.结果 对照组尿液中8-OHdG的排泄量（平均1.2μmo1/mol肌酐,n=36）与厨房里有排烟设备运转的厨师组相似（平均1.5 μmol/mol肌酐,n=45）.与对照组相比,接触油烟而排烟设备没有运转的厨师组,其尿8-OHdG的排泄量明显增加（平均2.3 μmol/mol 肌酐,n=18）.经多元回归分析,尿ln 1-OHP和ln 8-OHdG的水平仍然呈明显的正相关.结论 接触油烟中的多环芳烃（PAH）或其他化合物可能导致DNA氧化损伤.Objective To investigate the urinary excretion of a marker for oxidative DNA damage, 8-hydroxydeoxyguanosine （8-OHdG）, in different groups of cooks and different exposure groups, and to study the association between 8-OHdG and 1-hydroxypyrene（1-OHP）, a biological marker for PAH exposure. Methods Urine samples were collected from different groups of cooks （n=86） and from unexposed controls （n=36）, all were male with similar age and smoking habits. The health status, occupational history, smoking, and alcohol consumption 24 hours prior to sampling was estimated from questionnaires. The urinary samples were frozen for later analysis of 8-OHdG and 1-OHP by high performance liquid chromatography. Results Excretion in urine of 8-OHdG was similar for controls （mean 1.2 μmol/mol creatinine, n=36）, and for those who had been in the kitchen room with exhaust hood operation （mean 1.5 μmol/mol creatinine, n=45 ）. COF exposed cooks without exhaust hood operation had increased excretion of 8-OHdG （mean 2.3 μmol/mol creatinine, n= 18）. The differ-ence between this group and the unexposed controls was significant. The urinary levels of In 1-OHP and In 8-OHdG were still significantly correlated in a multiple regression analysis. Conclusion The exposure to PAH or possibly other compounds in COF may cause oxidative DNA damage.

关 键 词：多环碳氢化合物 芳香族 餐馆 DNA损伤 脱氧鸟苷 

分 类 号：R686[医药卫生—骨科学]