不同方法保存的猪软骨行异种移植的实验研究  

Experimental study on heterologous transplantation of pig cartilage preserved with different methods

作　　者：乔晨晖[1,2] 牛扶幼[1,2] 陈言汤[1,2] 刘林波 秦垦[1,2] 张建文[1,2] 陈静 

机构地区：[1]河南医科大学第一附属医院胸心外科 [2]河南医科大学第一附属医院整形外科

出　　处：《实用美容整形外科杂志》1999年第3期118-120,共3页Journal of Practical Aesthetic and Plastic Surgery

摘　　要：为了探索不同保存方法对猪肩胛软骨异种移植的影响，采用四种不同方法保存猪肩胛软骨，并按不同保存方法及保存时间分７组移植到日本大耳白兔背部皮下，每组５只动物，共３５只进行观察比较。①γ－射线辐射处理，４℃保存３个月为Ａ组，１个月为Ｂ组；②０．５％戊二醛磷酸缓冲液浸泡，４℃保存３个月为Ｃ组，１个月为Ｄ组；③深低温－８０℃保存３个月为Ｅ组，１个月为Ｆ组；④新鲜软骨为Ｇ组。移植术后每周一次活体检查软骨位置及大小。术后１周、１个月、３个月、６个月，每只动物取一块软骨行大体标本和光学显微镜观察，术后６个月取软骨标本行电子显微镜观察。实验结果显示：０．５％戊二醛浸泡、γ－射线辐射处理可降低软骨抗原性，４℃条件下保存以≤１个月为最佳时限，按软骨被吸收率大小，依次为Ｅ、Ｆ、Ａ、Ｂ、Ｇ、Ｃ、Ｄ组。结论：①猪肩胛软骨可作为异种移植代用材料的最佳供体之一；②猪肩胛软骨异种移植到家兔皮下，可存留达６个月；③本实验四种猪软骨保存方法，按异种移植后软骨抗原性降低和被吸收、变形程度轻重等情况相比较，以０．５％戊二醛浸泡保存方法最佳，γ－射线辐射处理次之；④０．５％戊二醛磷酸缓冲液４℃１个月是保存猪肩胛软骨供异种移植的最佳条件。To research the effects of different methods of preservation on xenografts of pig scapular cartilage, we have transplanted the pig scapular cartilages preserved with 4 different methods and storage time varied from 1 to 3 months under the back skin of 35 Japanese white rabbits. The cartilages for transplantation were divided into 7 groups according to the methods and periods of preservation. Group A, preserved with -ray irradiation at 4, for 3 months,Group B for 1 month. Group C, preserved in 0.5% glutaraldehyde phosphate buffer at 4 for 3 months; Group D, for 1 month. Group E, preserved at profound hypothermia -80 for 3 months; Group D, for 1 month. Group G, fresh cartilage. The sites and sizes of heterogafts were examined once a week after operation. Every heterogaft taken from each rabbit was evaluated grossly and microscopically at 1 week,1 month, 3 months and 6 months, respectively, after transplantation. Ultrastructural changes were observed 6 months after operation. The results indicated that the antigenicity of cartilage grafts preserved in 0.5% glutaraldehyde solution and processed with -ray irradiation was reduced. The optimal time for storage of cartilages was 1 month or less at 4. According to the rate of absorption of cartilage grafts, Group E, F, A, B, G, C, D were arranged at a progressive sequence. By comprehensive analysis it is concluded that: pig scapular cartilage is one of the optimal donors for heterograft; pig scapular cartilage heterografts remain intact for 6 months after being transplanted to the subcutaneous tissue of rabbits; by comparison of the 4 methods and according to the reduction of antigenicity, and the degrees of absorption and malformation after heterografts, it suggests that preservation of pig cartilages in 0.5% glutaraldehyde solution may be optimal and irradiati

关 键 词：猪 软骨保藏 异种移植 软骨移植 

分 类 号：R687.34[医药卫生—骨科学]