吴茱萸总生物碱对大鼠肝纤维化的防治作用  

Protective Effects of Total Alkaloids of Evodia Rutaecarpa against Carbon Tetrachloride Induced Liver Fibrosis in Rats

作　　者：杨冬梅[1,2] 李莉[3] 周禹[3] 马泽彬[3] 朱克克[3] 蒋磊[1,2] 

机构地区：[1]安徽医学高等专科学校附属医院 [2]安徽医学高等专科学校药理教研室,安徽合肥230022 [3]安徽中医学院中西医结合临床学院,安徽合肥230038

出　　处：《安徽中医学院学报》2010年第4期55-59,共5页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽医学高等专科学校自然科学研究基金项目(KJ2008X11)

摘　　要：目的研究吴茱萸总生物碱(total alkaloids ofEvodiarutaecarpa,TAE)对四氯化碳(carbon tetra-chloride,CCl4)致大鼠实验性肝纤维化的防治作用,探索其作用机制。方法采用40%CCl4皮下注射12周复制大鼠肝纤维化模型,检测各组大鼠血清丙氨酸转氨酶(alanine transaminase,ALT)、天冬氨酸转氨酶(aspartate transaminase,AST),层黏连蛋白(laminin,LN)、透明质酸(hyalurnic acid,HA)、Ⅲ型前胶原(precollagen typeⅢ,PCⅢ)、丙二醛(malonaldehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和肝组织中羟脯氨酸(hydroxyproline,Hyp)水平,并对肝脏进行病理学检查。结果模型组大鼠肝脏有明显胶原沉积、脂肪变性和肝纤维化,部分大鼠形成完整的假小叶;血清ALT、AST、MDA、SOD、LN、HA、PCⅢ和肝组织中Hyp含量明显高于正常组(P<0.05,或P<0.01)。给药10周末,TAE 50,100mg/kg组肝小叶结构明显改善,脂肪变性减轻,胶原纤维间隔减少,ALT、AST、MDA、LN、HA、PCⅢ及肝组织中Hyp水平明显下降。结论TAE 50,100 mg/kg灌胃给药对CCl4致大鼠实验性肝纤维化有保护作用,其作用机制与抗脂质过氧化有关。Objective To study preventive effects of total alkaloids of Evodia rutaecarpa （ TAE ） against carbon tetrachloride （Gel4） induced liver fibrosis in rats, and to explore its mechanism. Methods The rat model of liver fibrosis was induced by hypodermic injection of 40% CCl4 for 12 weeks, consecutively. Serum levels of alanine transaminase （ALT）, aspartate transaminase （AST）, laminin （LN）, hyalurnic acid （HA） , precollagen type Ⅲ （PCⅢ）, malonaldehyde （MDA）, and superoxide dismutase （SOD） as well as the level of hydroxyproline （Hyp） in liver tissues were assayed. The histopathologic changes of livers were observed using hematoxylin eosin staining. Results Changes of remarkable collagen accumulation, fatty degeneration and liver fibrosis were found in model rats, and pseudoloculus could be observed in some model rats. The serum levels of ALT, AST, MDA, SOD, LN, HA, and PCⅢ as well as the level of Hyp in liver tissues were significantly higher in the model group than those in the normal group （P〈0.05, or P〈0.01）. After treatment for 10 weeks, TAE 50, 100 mg/kg obviously alleviate liver pseudoloculus, and inhibited liver fibrosis and fatty degeneration, and decreased the serum levels of ALT, AST, MDA, LN, HA, PCⅢ and the level of Hyp in liver tissues. Conclusion TAE 50, 100 mg/kg have protective effects against CCl4 induced liver fibrosis in rats, its mechanism is related with effects against lipid peroxidation.

关 键 词：肝纤维化 抗氧化 吴茱萸总生物碱 

分 类 号：R657.31[医药卫生—外科学] R285.5[医药卫生—临床医学]