高效液相色谱法测定痛血康胶囊中重楼皂苷的含量  

Determination of paridis saponins in Tongxuekang capsule by HPLC

作　　者：蒋宗解 

机构地区：[1]福建汇天生物药业有限公司,三明365000

出　　处：《海峡药学》2010年第8期87-90,共4页Strait Pharmaceutical Journal

摘　　要：目的测定痛血康胶囊中重楼皂苷Ⅰ(C51H82O20)和重楼皂苷Ⅱ(C44H70O16)的含量。方法高效液相色谱法。使用Hypersil ODS2(4.6mm×150mm,5μm)色谱柱;流动相为乙腈-水(42∶58);流速:1.0mL·min-1;检测波长:210nm。结果在1.6～16.0μg浓度范围内,重楼皂苷含量与峰面积呈良好的线性关系。重楼皂苷Ⅰ的回归方程Y=116.14X-9.167,r=0.9998,加样回收率为99.17%,RSD=0.65%(n=5);重楼皂苷Ⅱ的回归方程Y=111.28X-5.267,r=0.9997,加样回收率为99.13%,RSD=0.54%(n=5)。结论本方法精密度和重现性均较好,测定结果准确,可用于痛血康胶囊中重楼皂苷Ⅰ和重楼皂苷Ⅱ的含量测定。OBJECTIVE To establish the determination method for Paridis saponin I （C51 H82 O20 ） and Paridis saponin Ⅱ （C44H70o16） in Tongxuekang capsule. METHODS HPLC.Hypersil ODS2（4.6mm×150mm,5μm） column was used; Acetonitrile-water （42：58） was used as a mobile phase;Flow rate was 1.0mL· min^-1; Detection wavelength was 210nm. RESULTS The detection range was 1.6--16.0μg , the Paridis saponins concentration and peak area was showed good linear relationship between. The Paridis saponin I regression equation Y= 116.14X-9. 167, r = 0.9998.The recovery of the added sample was 99.17%, RSD = 0.65 （n = 6）; The Paridis saponin Ⅱ regression equation Y = 111.28X-5.267, r = 0. 9997. The recovery of the added sample was 99.13%,RSD=0.54（n=6）.CONCLUSION The method showed good precision and reproducibility, the result is accurate, can be used for Paridis saponin I （C51H82O20） and Paridis saponin Ⅱ （C44H70O16） of Content determination in Tongxuekang capsule.

关 键 词：痛血康胶囊 重楼皂苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]