丙戊酸钠的血药浓度监测及合理用药分析  

Determination of valproic acid sodium blood drug concentration and analysis of the rationality

作　　者：乔丽曼[1] 崔九虎[1] 潘小梅[1] 

机构地区：[1]温州医学院附属第二医院,温州325027

出　　处：《海峡药学》2010年第8期236-239,共4页Strait Pharmaceutical Journal

摘　　要：目的建立人血清中丙戊酸钠浓度测定方法。方法以二氯甲烷为提取溶剂,ω-溴苯乙酮为衍生试剂,环己烷羧酸为内标,采用高效液相色谱法测定,色谱柱:Hypersil ODS-C18(4.6mm×250mm,5μm)不锈钢色谱柱,流动相:甲醇-水(75∶25),检测波长:248nm,流速:1.0mL·min-1。结果丙戊酸钠浓度在25～150μg·mL-1范围内苯甲酰甲基丙戊酸和苯甲酰甲基环己烷羧酸的峰面积比与丙戊酸钠浓度呈现良好线性关(R2=0.9953),日内和日间RSD<4.19%。结论该方法快速、简便、准确,适合于临床血药浓度监测。OBJECTIVE To establish an analysis method for the determination of sodium valprate in the serum. METHODS Determination was performed with HPLC with methanol：water （75：25） as the mobile phase ω-bromine acetophenone as the deriving agent and cyclohexanecarboxylic acid as the internal standard . The analytical column was Hypersil ODS-C18（4.6mm×250mm,5μm）stainless steel column, velocity： 1.0 mL·min^-1, detected at 248nm. RESULTS The linear rang of sodium valproic was 25-150μg·mL^-1 within R2 = 0.9953 . Both the relative standard deviation of within day and between day was less than 5.1%. CONCLUSIONS This method is rapid, simple , accurate and suitable for clinical drug concentration monitoring.

关 键 词：丙戊酸钠 高效液相色谱法 血药浓度监测 用药分析 

分 类 号：R969.4[医药卫生—药理学] R927.3[医药卫生—药学]