应用RAPD标记技术研究大花蕙兰种质资源亲缘关系  

The genetic relationships of Cymbidium hybridum germplasm resources by RAPD molecular markers

作　　者：施隆文[1] 汪霞[1] 卢佳[1] 

机构地区：[1]嘉兴职业技术学院生物与环境分院,浙江嘉兴314036

出　　处：《浙江农业学报》2010年第4期414-419,共6页Acta Agriculturae Zhejiangensis

基　　金：嘉兴市科技局资助项目(2008AY1002)

摘　　要：利用RAPD标记技术对来自2个企业的18个大花蕙兰品种进行了检测。结果表明：从50个10碱基随机引物中筛选出15个多态性高、重复性好的引物,共扩增出92条DNA条带,其中90条为多态性带,占97.8%,平均每个引物扩增多态性带6条。18个种质之间的相似系数变化范围为0.1975~0.6704,平均相似系数为0.4761;遗传距离变化范围为0.3296~0.8025,平均遗传距离0.5239。基于RAPD的扩增结果建立了UPGMA亲缘关系聚类图,18份种质在遗传距离0.5239处被划分为4个类群。供试的18份种质间的遗传亲缘关系与其花色和花枝类型存在一定的相关性。The random amplified polymorphic DNA marker（RAPD） was assessed to detect the genetic relationships among 18 Cymbidium hybridum cultivars from 2 companies.The results showed that primers were screened from 50 random decamer primers,and a total of 92 DNA bands were amplified,90 of which（97.8%） were polymorphic.The average number of polymorphic DNA bands amplified by each primer was 6.Genetic similarities among the cultivars ranged from 0.1975 to 0.6704 with an average value of 0.4761,and the genetic distance among those ranged from 0.3296 to 0.8025 with an average value of 0.5239.A dendrogram of genetic relationship which was constructed using UPGMA method showed that all the tested cultivars were classified into 4 cluster groups with the genetic distance coefficient of 0.5239.It revealed that the genetic relationships among tested cultivars were related with flower color and branch type to some extent.

关 键 词：大花蕙兰 亲缘关系 RAPD标记技术 

分 类 号：S682.31[农业科学—观赏园艺]