水稻‘日本晴’T-DNA突变体材料纹枯病抗性资源的筛选  

Identification of resistance resources to sheath blight in rice T-DNA mutant library

作　　者：王艳丽[1] 陈涛[1] 柴荣耀[1] 张震[1] 王教瑜[1] 孙国昌[1] 

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2010年第4期439-443,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅重点项目(2006C22018);浙江省重大科技攻关计划(2007C12905)

摘　　要：从‘日本晴’水稻T-DNA突变体库中挑选出的2 576个株系,首先利用撒施法进行了水稻纹枯病抗性鉴定,发现多数为高感材料,抗病及中抗株系共有36个,中感株系有180个。再利用牙签嵌入法对初筛选出的发病中感以下的216个株系进行进一步抗性鉴定,发现18个株系抗性表现较好;并在温室中利用微室法对这18个株系进行了进一步抗性鉴定,结果表明株系间抗性差异不显著,但其中14个株系抗性与野生亲本‘日本晴’差异显著(P<0.01)。Isolated from Nipponbare T-DNA mutant library,2 576 rice mutant lines were used to identify resistance resources to sheath blight（SB）.Most of them were high-susceptible to rice SB by broadcasting the mixture of rice hulls and unhulled grains infested with the pathogen,and only 36 lines reached moderate resistant or resistant level,180 lines were moderately susceptible.Further identification was carried out among 216 lines whose resistances were below the moderate level using insertion short toothpick infected with pathogen,the results showed there were 18 lines more resistant than the others among secondary screening lines.Final screening with micro-chamber inoculation to 18 lines in greenhouse was carried out and the results indicated there was no significant difference among different mutant lines,but there are 14 mutants whose resistant level were significantly higher than Nipponbare.

关 键 词：水稻纹枯病 立枯丝核菌 T-DNA突变体 抗性资源 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]