我国西南西北地区吸毒人群重组人类免疫缺陷病毒1型毒株的发现  

The identification of recombinant HIV 1 strains in IDUs in southwest and northwest China

作　　者：邵一鸣 赵峰[1,2,3] 杨维中[1,2,3] 张远志[1,2,3] 龚新昌 

机构地区：[1]卫生部艾滋病预防与控制中心 [2]四川省卫生防疫站 [3]新疆维吾尔自治区卫生防疫站

出　　处：《中华实验和临床病毒学杂志》1999年第2期109-112,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的寻找人类免疫缺陷病毒１型（ＨＩＶ１型）在中国可能的重组。方法从流行２种以上ＨＩＶ１亚型的地区收集ＨＩＶ感染者的血样。从ＰＭＣｓ中应用套式聚合酶链反应（ＰＣＲ）方法，对ＨＩＶ病毒的ｔａｔ和ｅｎｖ基因进行扩增，ＰＣＲ产物直接测序并进行序列分析。结果对中国Ｂ′亚型和Ｃ亚型流行区域收集的１４个ＨＩＶ１毒株进行序列分析，对ｅｎｖ基因进行测序后没有发现重组毒株的证据。对ｔａｔ基因的第一外显子进行序列分析时，１４个样品中的１０个样品发现了Ｂ′亚型和Ｃ亚型重组的ＨＩＶ１毒株。此外，四川省静脉吸毒者中发现了３个非重组的Ｂ′亚型毒株和１个非重组的Ｃ亚型毒株。结论首次在中国西南部的四川省和西部的新疆维吾尔自治区发现了Ｂ′亚型和Ｃ型的重组ＨＩＶ１毒株。相同的序列和重组方式，表明重组毒株具有相同的起源，同时表明这两个艾滋病流行区域密切相关。由于在新疆只发现了重组毒株，而在四川省则发现了Ｂ亚型、Ｃ亚型和Ｂ′／Ｃ重组毒株，重组很有可能发生在四川而不是新疆。Objective To search for possible recombination of HIV1 strains in China. Methods The blood samples were collected from the HIV infected individuals in areas where epidemic of more than two subtypes of HIV1 were found. The HIV env and tat genes were amplified by nested PCR from PBMCs and directly sequenced and analyzed. Results The sequence analysis were performed on 14 HIV1 strains collected in the subtype Band C epidemic areas of China. No evidence of recombination after sequencing of env was found. The recombnant HIV1 strains of subtype B and C were found in 10 of the 14 samples tested when the first exon of the tat gene was sequenced. In addition, three subtype B and one subtype C nonrecombinant HIV1 strains were found among IDUs in Sichuan Province. Conclusions The recombinant HIV1 strains of subtype B and C were firstly identified in Sichuan Province of southwest China and Xinjiang Autonomous Region of western China. Their identical sequences and recombinant patterns indicated the same origin of the recombinant strains, it strongly indicates a close correlation between these two HIV epidemic regions. Since no other subtype of HIV1 except the recombinant was found in Xinjiang, whereas both subtype B and C as well as B/C recombinant were found in Sichuan, the recombination was most probably happened in Sichuan Province rather than in Xinjiang.

关 键 词：HIV TAT基因 ENV基因 重组 亚型 

分 类 号：R392-33[医药卫生—免疫学] R373.9[医药卫生—基础医学]