中国人类免疫缺陷病毒(HIV-1)D亚型毒株gag、env和tat基因的序列分析  

Identification and characterization of HIV1 subtype D strain in China

作　　者：邢辉[1,2] 秦光明[1,2] 冯毅 刘刚[1,2] 潘品良[1,2] 范秀娟 邵一鸣 

机构地区：[1]中国预防医学科学院艾滋病参比实验室 [2]四川省卫生防疫站

出　　处：《中华实验和临床病毒学杂志》1999年第2期157-162,共6页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的通过对ＨＩＶ１毒株ｇａｇ、ｔａｔ、ｅｎｖ基因的序列分析，阐明Ｄ亚型ＨＩＶ１毒株已在中国出现。方法从１名四川非洲回国劳务人员ＨＩＶ感染者（ＳＣ９７１２）淋巴细胞（ＰＢＭＣ）中提取前病毒ＤＮＡ，使用套式ＰＣＲ方法分别扩增ＨＩＶ１的ｇａｇ基因区、ｅｎｖ基因的Ｃ２Ｖ３区和ｔａｔ基因的第一外显子。结果发现ＳＣ９７１２在ｇａｇ区、ｅｎｖ区和ｔａｔ区与国际标准Ｄ亚型毒株的基因距离最近，其中在ｇａｇ区与Ｖ１２０３和Ｋ３１的基因离散率分别为９９４％和９５９％，在ｔａｔ基因区与ＵＧ２７４Ａ的基因离散率只有７５６％，在ｅｎｖ区与ＭＡＬ的基因离散率是１３８６％。系统树分析表明，ＳＣ９７１２与国际标准Ｄ亚型毒株、ＶＩ２０３、Ｋ３１、ＭＡＬ及ＵＧ２７４和Ｚ２７６等非洲流行株聚在一起。结论非洲Ｄ亚型毒株已随着援外劳务人员的回国传入中国。Objective Through sequence analysis of HIV1 gag, tat and env regions , the subtype D HIV1 had been identified appering in China. Methods The blood was collected from HIV1 infected individual (SC9 712) in Sichuan Province. The peripheral blod mononuclear cells (PBMCs) were isolated from the blood by ficoll and the genomic DNA was extracted. The gag gene, the C2V3 of the env gene and the first exon of tat gene were amplified by nestedPCR and sequenced directly. Results The genetic distance respectively is 7.56% in tat gene between SC9 712 and UG274A, 9.59% and 9.94% in gag gene between SC9 712 and K31 and VI203, 13.86% in env gene between SC9 712 and MAL. On the phylogenetic tree the SC9 712 HIV1 strain clustered with the Africa subtype D strains UG274A, K31, VI203 and MAL. The above data demonstrated that SC9 712 was subtype D HIV1 strain. Epidemiological data showed that SC9 712 virus was from a person who had worked in Africa. Conclusion The results indicated that the person who came back from Africa had transmitted the subtype D of HIV1 from Africa to China.

关 键 词：HIV NESTED-PCR 序列分析 系统树分析 

分 类 号：R373.9[医药卫生—病原生物学]