羊驼皮肤中核糖体蛋白L34(RPL34)基因表达的分析  

Transcripts of Ribosomal Protein L34 Gene Expression in the Alpaca Skin

作　　者：张俊珍[1] 范瑞文[1] 李鹏飞[1] 白瑞[1] 朱芷葳[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《激光生物学报》2010年第4期479-482,487,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30671512);山西农业大学科技创新基金项目(2006010)

摘　　要：从羊驼皮肤cDNA文库中筛选到RPL34的克隆,经PCR鉴定并测序得知:一种克隆的大小为270 bp(命名为RPL34Ⅰ),另一种克隆的大小为470 bp(命名为RPL34Ⅱ)。序列分析发现,RPL34Ⅰ和Ⅱ在核苷酸水平上,5'UTR区不具有同源性,3'UTR区具有同源性;在氨基酸水平上,部分具有高度同源性;分子进化树分析表明二者具有不同的进化速率。此外,RPL34Ⅰ和Ⅱ对应于不同的转录本。通过以上结果分析,认为RPL34Ⅰ和RPL34Ⅱ二者同时在羊驼皮肤中表达,可能是RPL34的两个剪接体,发挥不同的生物学功能。RPL34 positive clones were screened from the cDNA library of alpaca skin. The sizes of the inserted fragments of clones w.ere tested by PCR and sequenced. The results showed that the sizes of the inserted fragments were 270 bp （ named as RPL34 I） and 470 bp （ named as RPL34 II）. Both of them were analyzed and found that they had homolog in 3＇ UTR, hut no homolog in 5＇ UTR on the nuclear acid lever, part of them had homolog in the amino acid encoded, they had different evolution rate, and they corresponded different transcripts. It was concluded by all those evidence that RPL34 I and RPL34 II were the possible splicing variants of RPL34gene. While being expressed in the alpaca skin at the same time, RPL34 1 and RPL34 II may play different biological roles.

关 键 词：核糖体蛋白L34(RPL34) 皮肤 剪接体 羊驼 

分 类 号：S824[农业科学—畜牧学]