山羊精子发生不同阶段的显微与超微结构观察  

Spermatogenesis in Goat Capra hircus:Light Microscopy and Electronic Microscopy Studies

作　　者：施力光[1] 荀文娟[1] 岳文斌[1] 杨茹洁[1,2] 张春香[1] 任有蛇[1] 雷福林[3] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]山西农业大学太原畜牧兽医学院,山西太原030024 [3]山西农业大学实验基地黎城县种羊场,山西黎城047006

出　　处：《激光生物学报》2010年第4期488-494,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30871794)

摘　　要：应用光镜和透射电镜技术研究山羊精子发生不同阶段各级生精细胞显微、超微结构及山羊精子分化成熟过程。结果表明:山羊精子发生经历了精原细胞、初级精母细胞、次级精母细胞、精子细胞及变态精子阶段发育成成熟的精子。精原细胞期核呈椭圆形,染色质凝集成团分布于核质中,线粒体开始出现;精母细胞期有高尔基体分布;精子细胞经过核质浓缩、线粒体迁移等过程发育成成熟精子,成熟的山羊精子头部细长,核质高度浓缩,中段膨大,线粒体丰富。线粒体、中心粒对精子变态发生起重要作用,同时观察到头部与中段脱落的畸形精子。The microstructure and ultrastructure of the spermatogenic cells during different period and the maturing progress of germ cells in goat were observed. The result showed that ： the spermatogenesis of goats can be divided into several phases, as spermiogonium, primary spermatocyte, second spermatocyte, spermatid and spermatozoid. Spermatogonia contained a large oval nucleus with dense chromatin in the center and mitochondria were found. Golgi complex can be found during the spermatocytes stage. Spermatid altered into spermatozoa since caryoptasm and chromatin concentration and mitochondria redistribution. The mature spermatozoa consist of a rod-shaped and highly-condensed nucleus, an inflated midpiece and abundant mitochondria. Mitochondria centriole played an important role in speimatogenesis. At the same time, the teratosperms spitted in nucleus and midpieces can be observed.

关 键 词：山羊 精子发生 精原细胞 精母细胞 精子细胞 精子 显微结构 超微结构 

分 类 号：Q954.43[生物学—动物学] S827[农业科学—畜牧学]