肺特灵Ⅵ号对博莱霉素诱导的小鼠肺纤维化的干预作用及其机制  

Effect of Fei-te-ling Ⅵ on Bleomycin-induced Mice Pulmonary Fibrosis and Its Mechanisms

作　　者：郅玲然[1,2] 冯一中[1] 蒋小岗[3,2] 顾振纶[3,2] 高燕[3,2] 贺海彪[3,2] 郭次仪[2] 

机构地区：[1]苏州大学医学部病理学与病理生理学系,江苏苏州215123 [2]苏州中药研究所,江苏苏州215007 [3]苏州大学医学部药理学系,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2010年第3期470-472,490,F0002,共5页Suzhou University Journal of Medical Science

基　　金：江苏省中医药管理局资助项目(HZ07088);香港保健协会资助项目(20070906-10HK);苏州市科技局资助项目(SZ08008)

摘　　要：目的观察肺特灵Ⅵ号对博莱霉素诱导的小鼠肺纤维化的干预作用。方法采用气管内注入博莱霉素法制备小鼠肺纤维化模型。给药28d后处死,计算肺系数;观察肺脏病理形态学改变,并对肺泡炎和肺纤维化程度进行评分;测定小鼠肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)与羟脯氨酸(HYP)等生化指标。结果与模型组相比,肺特灵Ⅵ号各剂量组可明显增加小鼠体质量,降低肺系数,减轻肺泡炎与肺纤维化程度,提高肺组织SOD活性与T-AOC,降低MDA与HYP含量(P<0.05或<0.01)。结论肺特灵Ⅵ号对博莱霉素诱导的小鼠肺纤维化有一定的防治作用。Objective To investigate the protection of Fei-te-ling Ⅵ（FTL Ⅳ） on bleomycin-induced mice pulmonary fibrosis.Methods Models were reproduced by intratracheal injection of bleomycin.After continully administrated of 28 d,model animals in each group were sacrificed,and lungs were havested for investigating the changes of lung index,pathomorphological changes by HE staining.The extent of plumonary alveolitis and fibrosis were evaluated by Szaopiel＇s method;SOD,MDA,HYP and T-AOC in lung tissue were analyzed by biochemical detection.Results Contrasting with the model group,FTL Ⅵ could significantly increase weights of mice,depress lung index and the extent of lung alveolitis and fibrosis,FTL Ⅵ could significantly increase SOD,T-AOC and reduce the content of HYP,MDA in lung tissue（P0.05 or 0.01）.Conclusion FTL Ⅵ might have some protective effects on bleomycin-induced mice pulmonary fibrosis.

关 键 词：肺特灵Ⅵ号 博莱霉素 肺纤维化 小鼠 

分 类 号：R563.905[医药卫生—呼吸系统]