虎杖蒽醌化合物抗CVB_3病毒的实验研究  

Experimental Research on Effects of Anticoxsackie B 3 Virus of Anthraquinones of Rhizoma Polygoni Cuspidati

作　　者：王志洁[1] 

机构地区：[1]湖北医科大学病毒研究所

出　　处：《成都中医药大学学报》1999年第2期41-43,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：湖北省自然科学基金

摘　　要：目的：为了解虎杖蒽醌化合物晶Ⅰ、晶Ⅳ抗ＣＶＢ３病毒的药效，从而明确虎杖的抗病毒性能并开发利用。方法：以病毒唑为阳性对照，利用空斑减数实验进行研究。结果：在ＨＥＰ２细胞系统中，晶Ⅰ对ＣＶＢ３直接杀灭、增殖抑制、感染阻断的ＥＤ５０分别为１．５４μｇ／ｍＬ、８．３４μｇ／ｍＬ、３．０６μｇ／ｍＬ，晶Ⅳ的相应系数分别为１．７９μｇ／ｍＬ、７．０５μｇ／ｍＬ、３．１７μｇ／ｍＬ，而病毒唑的相应系数分别为６．２６μｇ／ｍＬ、７．５５μｇ／ｍＬ、７．５０μｇ／ｍＬ。结论：晶Ⅰ对ＣＶＢ３的治疗指数（ＴＩ）为病毒唑对ＣＶＢ３的ＴＩ的４．７、１．０、２．９倍；晶Ⅳ对ＣＶＢ３的治疗指数（ＴＩ）为病毒唑对ＣＶＢ３的ＴＩ的２７．５、８．４、１８．８倍，提示晶Ⅰ、晶Ⅳ的抗ＣＶＢ３方面有一定优势。:Objective:Rhizoma polygoni cuspidati is a chinese medicinal herb with large antiviral range, in order to understand and exploit it's antiviral properties, the authors extracted anthraquinones of it, that is cryatalline Ⅰ and crystalline Ⅳ, and observed effects of anticoxsackie B 3 virus of them. Methods:Used ribavirin as positive control, and by way of plaque reduction assays, carry out this research. Results:In HEP 2 cell system, the ED 50 s of direct virucidal activity, the inhibition effects on the multiplication and infection of coxsackie B 3 virus are 6.26μg/mL, 7.50μg/mL, 1.75μg/mL respectively, the corresponding figures of crystalline Ⅰ are 1.54μg/mL, 8.34μg/mL, 3.06μg/mL separately, and the corresponding figures of crystalline Ⅳ are 1.79μg/mL, 7.05μg/mL, 3.17μg/mL repectively. Conclusion:The treatedment indices (TI) of crystalline Ⅰ to CVB 3 are 4.7, 1.0, 2.9 times of ribavirin TIs, the TIs of crystalline Ⅳ to CVB 3 are 27.5, 8.4, 18.8 times of ribavirin TIs, they should be worth exploitation.

关 键 词：虎杖蒽醌化合物 柯萨基病毒 中医药疗法 实验 

分 类 号：R259.125[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]