利用复合生物支架材料构建全生物型小口径组织工程血管的研究  

Construction of Completely Biological Tissue-Engineered Small-Diameter Blood Vessel Based on a Biological Hybrid Scaffold

作　　者：李庆春[1] 杨大平[1] 刘国锋[1] 徐学武[1] 何志娟[2] 赵震宇[1] 

机构地区：[1]哈尔滨医科大学附属第二医院整形外科,黑龙江哈尔滨市150086 [2]哈尔滨医科大学附属第一医院妇产科,黑龙江哈尔滨市150010

出　　处：《中国康复理论与实践》2010年第8期752-754,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中国博士后科学基金项目(20100471098);黑龙江省青年科学基金项目(QC2009C38);哈尔滨市科技创新人才研究专项基金项目(2010RFQXS081);黑龙江省卫生厅科研基金项目(2009-124);哈尔滨医科大学附属第二医院院博士科研基金项目(BS2009-18)

摘　　要：目的利用复合纤维蛋白胶生物支架材料和血管细胞成分体外构建具有完整中膜及内皮层的全生物型小口径组织工程血管。方法体外培养扩增猪主动脉血管内皮细胞及平滑细胞,将血管平滑肌细胞与猪纤维蛋白胶混合,均匀复合于犬颈总动脉脱细胞基质材料外表面,经培养后将血管内皮细胞悬液种植到基质材料的内表面,构建全生物型小口径组织工程血管。通过组织学染色观察组织工程血管的组织学结构,扫描电镜观察血管的内外表面结构。结果组织学染色结果表明构建的小口径组织工程血管具有完整的内膜层及中膜层结构;扫描电镜结果显示组织工程血管内表面覆盖着完整的内皮细胞层,血管的外表面可见分层排列的血管平滑肌细胞。结论利用纤维蛋白胶血管细胞混合液复合犬颈总动脉脱细胞基质可以构建出具有完整中膜及内膜层的全生物型小口径组织工程血管。Objective To prepare completely biological tissue-engineered small-diameter blood vessel based on a biological hybrid scaffold.Methods Endothelial cells and smooth muscle cells were isolated from the porcine aorta and expanded in vitro. Mixture of smooth muscle cells and porcine fibrin was prayed coating on the decellularized canine carotid artery. Then,the inner surface of the decellularized artery was seeded with the endothelial cells to construction of completely biological tissue-engineered small-diameter blood vessel. The tissue-engineered blood vessel was evaluated with Hematoxylin and Eosin （HE） staining and scanning electron microscopy.Results Histology examination revealed that the completely biological tissue-engineered small-diameter had intact media and intima. Scanning electron microscopy examination confirmed that the inner surface of tissue-engineered blood vessel was covered with intact monolayer endothelial cells and the external surface was covered with multilayer smooth muscle cells.Conclusion The completely biological tissue-engineered small-diameter with intact media and intima was prepared using mixture of blood vessel cells and porcine fibrin on the decellularized canine carotid artery.

关 键 词：血管 组织工程 生物支架材料 纤维蛋白胶 细胞外基质 

分 类 号：R318.11[医药卫生—生物医学工程]