西陵知母中甾体皂苷的分离与鉴定  

ISOLATION AND IDENTIFICATION OF STEROIDAL SAPONINS FROM ANEMARRHENA ASPHODELOIDES BUNGE.

作　　者：洪永福[1,2] 张广明[1,2] 孙连娜[1,2] 韩公羽 计国桢[1,2] 

机构地区：[1]第二军医大学药学院 [2]中科院上海有机化学研究所

出　　处：《药学学报》1999年第7期518-521,共4页Acta Pharmaceutica Sinica

摘　　要：目的：从新鲜西陵知母（ＡｎｅｍａｒｒｈｅｎａａｓｐｈｏｄｅｌｏｉｄｅｓＢｕｎｇｅ．）根茎中分离出有生理活性的化学成分—甾体皂苷。方法：用柱色谱法分离纯化，通过理化方法及光谱分析（ＵＶ，ＩＲ，１ＨＮＭＲ，１３ＣＮＭＲ，ＥＩＭＳ，ＥＳＩＭＳ，ＨＭＢＣ，ＨＭＱＣ）鉴定化学结构。结果：从新鲜西陵知母根茎的乙醇提取物中分离得到西陵知母皂苷Ａ与Ｂ，其化学结构分别为萨尔萨皂苷元３ＯβＤ葡糖基（１→２）ＯβＤ半乳糖苷和萨尔萨皂苷元３ＯβＤ葡糖基（１→３）ＯβＤ葡糖基（１→２）ＯβＤ半乳糖苷。结论：西陵皂苷Ｂ为一新化合物。AIM: To isolate and elucidate the chemical structures of he steroidal saponins possessing physiological activity from the fresh rhizoma of Anemarrhena asphodeloides Bunge. (Xilingzhimu). METHODS: The constituents were isolated and purified by repeated column chromatography on silica gel. The molecular structures were elucidated on the basis of chemical evidences and spectral analysis (UV, IR, EI MS, EIS MS, 1HNMR, 13 CNMR, HMBC and HMQC). RESULTS: Two steroidal saponins, xilingsaponin A and B were obtained from the fresh rhizoma of Xilingzhimu. They were elucidated as sarsasapogenin 3 O β D glucopyranosyl (1→2) β D galactopyranoside and sarsasapogenin 3 O β D glucopyranosyl (1→3) β D glucopyranosyl (1→2) β D galactopyranoside respectively. CONCLUSION: Xilingsaponin B is a new compound.

关 键 词：知母 中药 甾体皂苷 西陵皂苷A 西陵皂苷B 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]