醋酸甲羟孕酮的极谱行为及其分析应用  

POLAROGRAPHIC BEHAVIOUR OF MEDROXYPROGESTERONE ACETATE AND ITS ANALYTICAL APPLICATION

作　　者：亢晓峰[1] 杨涛[1] 宋俊峰[1] 

机构地区：[1]西北大学电分析化学研究所

出　　处：《药学学报》1999年第7期530-534,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：目的：研究醋酸甲羟孕酮（ＭＰＡ）的电极反应机理，拟定了测定ＭＰＡ的单扫描示波极谱法。方法：用单扫描示波极谱法、快速扫描循环伏安法、恒电位电解法、紫外分光光度法等多种技术进行研究。结果：在醋酸盐缓冲溶液中，ＭＰＡ的Ｃ＝Ｃ双键首先发生单电子单质子还原，产生了中间体自由基ＨＭＰＡ·。随后ＨＭＰＡ·可以单电子单质子方式进一步还原，形成产物Ｈ２ＭＰＡ；同时也可以与中性ＭＰＡ分子形成二聚体自由基ＨＭＰＡ·ＭＰＡ·。在０２ｍｏｌ·Ｌ－１ＨＡｃ—ＮａＡｃ缓冲液中，ＭＰＡ还原波的二阶导数峰电流ｉＰ″与其浓度ＣＭＰＡ在２０×１０－６～６０×１０－５ｍｏｌ·Ｌ－１范围内成线性关系，相关系数γ＝０９９８。结论：证实ＭＰＡ的还原过程有中间体自由基参与。AIM: To study the polarographic behaviour and reduction mechanism of medroxyprogesterone acetate (MPA) in acetate buffer solution. A new method for the determination of MPA was established by linear sweep polarography. METHODS: Fast scan cyclic voltammetry, controlled potential electrolysis, linear sweep polarography and ultraviolet visible spectrophotometry were used in the present research. RESULTS: The C=C bond of MPA first undergoes a 1 e, 1H + reduction to form a radical HMPA ·, then the further reduction of HMPA · in 1 e, 1H + process is simultaneous with the dimerization reaction between HMPA · and the neutral molecule MPA. In 0 2 mol·L -1 HAc NaAc (pH 5 0) buffer solution, the reduction wave ( E P= -1 33 V , vs.SCE) of MPA can be used for analytical purpose. The second order derivative peak current of MPA is linear with the MPA concentration over the range of 2 0×10 -6 ～6 0×10 -5 mol·L -1 . The linear regression equation is i P″(nA)=5 81+6 8×10 5 C MPA (mol·L -1 ), and the correlation coefficient γ=0 998. The detection limit is 8 0×10 -7 mol·L -1 .CONCLUSION: The characteristics of the polarographic reduction of steroids was elucidated. The process of reduction was the two step transmission process of electric charge with the intermediate free radical.

关 键 词：醋酸甲羟孕酮 单扫描 示波极谱法 

分 类 号：R977.12[医药卫生—药品]