抑制肝癌HepG2细胞N-Ras基因表达对放射敏感性的影响  

A study on interference of N-Ras by RNA to enhance radiosensitivity of hepatoma carcinoma cell HepG2

作　　者：张洪波[1] 张红雁[1] 王宏梅[2] 罗文广[1] 陈龙华[2] 

机构地区：[1]安徽省立医院肿瘤放射治疗科,安徽合肥230000 [2]南方医科大学南方医院肿瘤放射治疗科,广东广州510515

出　　处：《蚌埠医学院学报》2010年第9期889-892,共4页Journal of Bengbu Medical College

基　　金：国家自然科学基金资助项目(30772530);安徽省卫生厅医学科研资助项目(09B109)

摘　　要：目的:探讨RNA干扰(RNAi)N-Ras基因增强人肝癌HepG2细胞的放射敏感性。方法:通过构建干扰N-Ras基因siRNA,采用免疫组织化学、荧光定量RT-PCR、Western blot、MTT法等观察干扰前后的肝癌HepG2细胞RNA水平、蛋白水平、生长情况等变化。用细胞克隆计数实验检测放射敏感性变化。结果:肝癌HepG2细胞株干扰后mRNA、蛋白、免疫组织化学、生长情况均显著改变,HepG2细胞RNAi前后增敏比SER=1.10。结论:RNAi肝癌HepG2细胞N-Ras基因可以达到放射增敏的目的。Objective：To investigate the radiosensitivity of silencing N-Ras by RNA interference in Hepatoma carcinoma cell HepG2.Methods：N-Ras RNA interference（RNAi） vector was constructed by using pcDNA 6.2-GW/EmGFP-miR plasmid.The RNAi effect was detected by RT-PCR,Western blot,immunohistochemisty and MTT method.Survival curves for each cell line were obtained by measuring the colony forming abilities of irradiated cell populations.Results：After silencing the N-Ras by RNAi,the expression of N-Ras mRNA,N-Ras protein and the survival of hepatoma carcinoma cell HepG2 line were significant difference in statistics.Conclusions：RNAi targeting silenced N-Ras may increase the radiosensitivity of hepatoma carcinoma cell HepG2 line.

关 键 词：放射敏感性 N-RAS基因 RNA干扰 肝肿瘤 

分 类 号：R814.2[医药卫生—影像医学与核医学]