全自动生化分析仪分析项目间试剂交叉污染及避免方法  

The methods to avoid cross-contamination in automatic biochemical analyzer

作　　者：葛鑫[1] 刘刚[2] 

机构地区：[1]蚌埠医学院第一附属医院检验科,安徽蚌埠233004 [2]安徽省蚌埠市第三人民医院检验科,233000

出　　处：《蚌埠医学院学报》2010年第9期943-944,共2页Journal of Bengbu Medical College

摘　　要：目的:探讨全自动生化分析仪分析项目间试剂交叉污染及避免的对策。方法:用质控血清分别先测血清碱性磷酸酶(ALP)后测Mg2+(A组);先测淀粉酶(AMY)后测Mg2+(B组);用质控血清测定Mg2+(对照组)。用去离子水当样品测1次ALP后,再连测10次Mg2+,用质控血清替代去离子水当样品同样操作(C组);将ALP换成AMY进行同样操作(D组)。结果:A、B两组污染率差为9.93%和10.13%,ALP和AMY试剂对Mg2+测定有交叉污染。结论:检验人员熟悉仪器的操作及性能,在仪器项目顺序安排时尽量将有污染的项目隔开,按一定的顺序安排项目,可以避免试剂间的化学污染。Objective：To discuss the treatment of avoiding the different item′s cross-contamination of automatic biochemical analyzer.Methods：There were divided into five groups： group A： the contents of alkaline phosphatase（ALP） in patients′ serum with quality control serum were verified,then Mg2＋（magnesia ion） was verified;group B：firstly amylase（AMY） then Mg2＋ were verified;matched group： just assess Mg2＋;group C： the deionizer water was considered as sample,firstly assess the content of ALP in deionizer water,then assess Mg2＋ ten times,afterward do the same processes which using quality control serum instead of deionizer water;group D：Use AMY instead of ALP,and do above-mentioned procedure.Results：Group A and group B the pollution rate were 9.93% and 10.13%.The cross-contamination was happened in assessment of Mg2＋ as affected by reagent ALP and AMY.Conclusions：Medical technologists must be familiar with the manipulation and function of machines.When arranging the order of machines′ testing items,it is needed to separate these items which has cross-contamination as far as possible.With the specific order,chemical pollution in different reagents can be reduced.

关 键 词：诊断 实验室 碱性磷酸酶 淀粉酶 

分 类 号：R446[医药卫生—诊断学]