培养处理与冷冻保存的活体异体肌腱移植的实验研究  

EXPERIMENTAL STUDY OF CULTURED AND CRYOPRESERVED VIABLE TENDON ALLOGRAFT

作　　者：唐林俊[1] 程国良[1] 陈秉礼[1] 方光荣[1] 杨志贤[1] 林彬[1] 

机构地区：[1]解放军第401医院

出　　处：《解放军医学杂志》1999年第3期196-199,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：用脱氧鸟苷培养处理鸡屈趾深肌腱５天，无创冻存于液氮贮存器（－１９６℃）中３个月，使用前将冻存腱在４０℃溶液中融解并洗去腱中吸收的二甲基亚砜。即刻作同种异体移植，自体移植对照。混合淋巴细胞培养实验及腱细胞活力测定证实，脱氧鸟苷培养及无创冻存处理肌腱显著降低了肌腱抗原性并保留了肌腱活性。趾主动屈曲功能，大体、显微及超微观察，羟脯氨酸含量测定及生物力学分析等结果均显示，培养及冷冻处理的异体移植肌腱与自体移植肌腱差异无显著性意义。说明脱氧鸟苷培养及无创冻存处理肌腱可显著降低肌腱抗原性并保留了肌腱活性。培养处理及冷冻保存的肌腱异体移植可达到与自体移植相同的良好效果。Flexor digitorum profundus tendons of chickens were cultured in the presence of deoxyguanosine(dGua) for 5 days, then cryopreserved in liquid nitrogen (-196) without affecting their viability. After three months of liguid nitrogen storage, they were thawed in 40 water, and dimethyl sulfoxide was washed away just before use. They were then transplanted into allogeneic recipients, using autografts as controls. Mixed lymphocyte culture and determination of tendon cell activity suggested that the antigenicity of dGuacultured and cryopreserved tendons was significantly attenuated and the cultured and cryopreserved tendons were viable. Digital active flexion function, the macroscopic and microscopic and ultrastructural observations, the hydroxyproline levels and the biomechanics analysis confirmed tAuthor hat the differences were not significant between the cultured and cryopreserved homografts and the autografts. Conclusion:(1)the antigenicity of dGuacultured and cryopreserved tendons could be significantly decreased and the viability of tendonocytes be preserved.(2) Transplantation of cultured and cryopreserved tendon homografts were as successful as that of the autografts.

关 键 词：脱氧鸟苷 冷冻保存 肌腱移植 异体移植 

分 类 号：R622.2[医药卫生—整形外科]