支气管哮喘患儿血清总IgE、TNF-α检测及其临床意义  

The clinical value of serum total IgE and serum TNF-α in asthmatic children

作　　者：吴红梅[1] 于莹[1] 张莉[1] 施玉梅[1] 陆志芳[1] 

机构地区：[1]南京医科大学附属南京第一医院儿科,江苏南京210006

出　　处：《中国实验诊断学》2010年第8期1244-1246,共3页Chinese Journal of Laboratory Diagnosis

基　　金：南京医科大学科技发展基金项目(07NMUM112)

摘　　要：目的探讨支气管哮喘患儿血清总IgE、TNF-α检测的临床意义。方法 2008年1月至8月,分别采用CLIA法、RIA法测定43例支气管哮喘患儿急性发作期血清总IgE水平、TNF-α水平,并与41例正常健康儿童对照组比较。观察组支气管哮喘患儿经治疗后症状体征缓解,跟踪随访8个月,记录哮喘的急性发作次数及严重程度。结果支气管哮喘患儿急性发作期血清总IgE、TNF-α水平分别高于对照组(t′分别为2.72、2.23),差异有统计学意义(P<0.05)。血清总IgE水平越高,支气管哮喘患儿急性发作次数越多,呈正相关(r=0.502,P<0.01);血清TNF-α水平越高,支气管哮喘患儿发作程度越重(r=0.484,P<0.01),平均发作程度(8个月内)越重(r=0.458,P<0.01),分别统计呈正相关。结论支气管哮喘患儿急性发作期血清总IgE、TNF-α水平升高,且与其预后有相关性:血清总IgE水平越高,急性发作次数越多;血清TNF-α水平越高,发作程度越重。Objective To explore the clinical value of serum total IgE and serum TNF-α in asthmatic children. Methods The levels of serum total IgE and serum TNF-α in 43 children with acute asthmatic attack （study group） and 41 normal children （control group） were measured （total IgE ： by CLIA, TNF-α： by RIA）. Then the study group were followed up for 8 months. Results The levels of serum total IgE and serum TNF-a in the study group were higher than those in the control group（ t＇ = 2.72,2.23, P 〈 0.05）. Significant positive linear correlations were observed for the study group between the level of serum total IgE and the times of acute asthmatic attacks （in 8 months） （ r = 0. 502, P 〈 0.01）, TNF-α and the degree of the acute asthmatic attack（ r = 0.484, P .〈 0.01）, TNF-α and ＇the average degree of acute asthmatic attacks（ in 8 months） （ r = 0.458, P 〈 0.01 ）. Conclusion The levels of serum total IgE and serum TNF-a in children with acute asttmaatic attack were higher than those in noroaal children. And there was a correlation between the serum total IgE and serum TNF-α levels with the prognosis in children with acute asthmatic attack.

关 键 词：支气管哮喘 总IGE TNF-Α 

分 类 号：R725.6[医药卫生—儿科]