广西瑶族与汉族人多瘤病毒感染率的调查研究  

Prevalence of BK Virus infection in healthy individuals among Yao minority in Guangxi

作　　者：龙柳艳[1] 

机构地区：[1]广西医科大学第四附属医院广西柳州市工人医院,广西柳州545005

出　　处：《中国实验诊断学》2010年第8期1257-1259,共3页Chinese Journal of Laboratory Diagnosis

基　　金：柳州市科技三项经费资助项目(柳科字2007021317)

摘　　要：目的研究广西瑶族与汉族健康成人外周血白细胞(PBLs)中人多瘤病毒(BKV)感染的发生率。方法采集广西瑶族及汉族健康人外周血标本各500份,提取淋巴细胞基因组DNA,用巢式PCR方法扩增BKV的保守区编码序列,统计分析不同民族、年龄、性别组BKV-DNA检出率。结果 1 000份健康人PBLs样品中BKV感染的发生率为61.3%,不同民族、性别、年龄组之间无显著性差异(P>0.05)。结论 BKV在广西瑶族及汉族健康人PBLs中存在较高的感染率,本研究证实了PBLs是BKV在体内的潜伏细胞及传播载体,应加强对BKV条件致病性的认识,积极防治、减少BKV相关的移植肾病(BKVN)的发生率。Objective To investigate the prevalence of BK virus in peripheral blood leukocytes （PBI_s）of healthy individuals a- mong Yao minority in Guangxi so as to describe the transmission of BKV in human body. Methods DNA in peripheral blood leuko- cytcs （from 2500 blood samples of Yao and Han healthy adult individuals between January 2006 and May 2008 was extracted and BKV DNA was examined by nested polymerase chain reaction. The BKV-DNA detection rates were statistically analyzed among the groups of different minorities, ages and sex. Results In the 2500 peripheral leukocytes samples, the detection rate of BKV-DNA was 60.6%. There was no significant difference in nationalities, sex or age distribution （ P 〉 0.05）. Conclusion It shows a high prevalence of BKV in peripheral blood leukecytes of Yao and Han healthy individuals in Guangxi. This study shows that PBLs are the harbors and the transmission carriers for BKV. Awareness of the opportunistic pathogenicity of BKV should be improved so as to decrease and prevent the incidence of BKV nephrosis.

关 键 词：多瘤病毒BK 外周血白细胞PBLs 感染率 广西瑶族 

分 类 号：R181.3[医药卫生—流行病学]