地衣芽孢杆菌De株对凡纳滨对虾粪便的降解效果  

Degradation effects of Bacillus licheniformis strain De on shrimp (Litopenaeus vannamei) dejecta

作　　者：曹煜成[1] 李卓佳[1] 林小涛[2] 杨莺莺[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]暨南大学水生生物研究所,广东广州510632

出　　处：《热带海洋学报》2010年第4期125-131,共7页Journal of Tropical Oceanography

基　　金：国家"十一五"支撑项目(2006BAD03B0106;2006BAD09A07);中央级科研院所基本科研专项(2007ZD01);广东省科技计划(2007A020400001);广东省重大科技兴渔项目(A200899A06);公益性农业行业专项(nyhyzx07-042\200803012);现代农业(虾)产业技术体系建设专项(nycytx46);广东省鱼病防治专项(2130108)

摘　　要：采用独立分析法,研究地衣芽孢杆菌Bacillus licheniformis De株降解凡纳滨对虾Litopenaeus vannamei粪便的效果。所收集对虾粪便先经冻干、粉碎,再溶解于灭菌海水中,分别在不同温度(16、21、26和31℃)、虾粪含量(5、10和20mg·L-1)、芽孢杆菌初始添加量(1、2.5、5和10mg·L-1)等条件下分析地衣芽孢杆菌De株对对虾粪便溶解液中的NH3—N、NO2-—N、NO3-—N、PO4-—P及COD的降解效果。结果表明,地衣芽孢杆菌De株可有效降低样品中COD和NO3-—N的含量,其平均降解率分别高于60%和50%,而NH3—N、NO2-—N、及PO43-—P等的浓度则不断升高;总体而言,地衣芽孢杆菌De株在26—31℃,初始添加量5mg·L-1时对对虾粪便具有较好的降解效果(p<0.05);当粪便溶解液浓度大于10mg·L-1时组间各项参数的差异不显著(p>0.05)。The degradation activity of Bacillus licheniformis De strain on shrimp dejecta was analyzed by an independent analytical method.The interference effects are avoidance,including biologic or environmental factors in ecosystem of aquaculture ponds.Dejecta of Litopenaeus vannamei was collected,dehydrofreezed,and dissolved in disinfected sea water after comminuted as powder.The degradation effects of NH3—N,NO2-—N,NO3-—N,PO4-—P and COD in the dejecta solution were studied at different temperature（16,21,26 and 31℃）,dejecta（5,10 and 20mg·L-1） and Bacillus concentration（1,2.5,5 and 10mg·L-1）.The results show that concentrations of both COD and NO3-—N decreased;their average degradation rates respectively exceeded 60% and 50%,but the concentrations of NH3—N、NO2-—N and PO4-—P all continually increased.There were significant effects（p0.05） of temperature and of all parameters,with optimum conditions respectively at 26—31oC and Bacillus concentration of 5mg·L-1.The differences of each two groups were insignificant（p0.05） as the dejecta concentration exceeded 10mg·L-1.

关 键 词：地衣芽孢杆菌Bacillus LICHENIFORMIS 凡纳滨对虾Litopenaeus vannamei 粪便 降解效果 

分 类 号：S963.2[农业科学—水产养殖] S942.3[农业科学—水产科学]