利用溴酸钠-苹果酸-[CuL](ClO_4)_2-硫酸化学振荡体系分析测定酪氨酸  

Determination of tyrosine based on NaBrO_3-Malic acid-[CuL](ClO_4)_2-H_2SO_4 oscillating system

作　　者：沈慧[1] 张祖德[1] 胡刚[2] 

机构地区：[1]中国科学技术大学化学系,安徽合肥230026 [2]安徽大学化学系,安徽合肥230039

出　　处：《中国科学技术大学学报》2010年第7期686-693,共8页JUSTC

基　　金：安徽省教育厅重点科研项目(KJ2008A118)资助

摘　　要：利用不同浓度的酪氨酸对溴酸钠-苹果酸-[CuL](ClO4)2-硫酸化学振荡反应体系的扰动,得到一种新的定量分析测定酪氨酸的方法,其中[CuL](ClO4)2的L为5,7,7,12,14,14-六甲基-1,4,8,11-四氮杂环十四-4,11-二烯.研究结果表明,当不同浓度的酪氨酸加入到振荡反应体系时,振荡振幅的改变值ΔAm与酪氨酸浓度的对数值lg{[Tyr]0}之间呈良好的线性关系,线性范围为1.235×10-6～2.677×10-4mol/L,相关系数r=0.99918;振荡周期的改变值ΔT与酪氨酸浓度的对数值lg{[Tyr]0}呈良好的线性关系,线性范围为7.444×10-7～4.534×10-4mol/L,相关系数r=0.99361.此外还研究了反应温度、搅拌速度和组成浓度对该振荡反应的影响,同时初步探讨了酪氨酸扰动化学振荡的机理.A new quantitative analytical method for the determination of tyrosine by the perturbation caused by different amounts of tyrosine on the NaBrO3-Malic acid-[CuL]（ClO4）2-H2SO4oscillating chemical system is reported.The unsaturated ligand L in the complex [CuL]（ClO4）2is 5,7,7,12,14,14- hexemethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene.When different concentrations of tyrosine is injected into the oscillating chemical system,the mutation of the amplitudeΔAmfits a linear relationship to the logarithm of the concentration of tyrosine lg{[Tyr]0}in the range 1.235×10^-6～2.677×10^-4 mol/L very well.The correlation coefficient is 0.999 18.The experimental results also show that there is the linear relationship between the change in the oscillating periodΔT of the chemical system and the logarithm of the concentration of tyrosine lg{[Tyr]0}in the range 7.444×10^-7～4.534×10^-4 mol/L.The correlation coefficient is 0.993 61.The effect of influential variables,such as temperature,stirring rate and the concentration of components on the oscillating system are studied.Some aspects of the probable mechanism of perturbation of tyrosine on the oscillating chemical system are also discussed.

关 键 词：化学振荡 四氮杂大环铜配合物 酪氨酸 扰动 

分 类 号：O654.1[理学—分析化学]