全氟辛磺酸对于爪蟾的甲状腺激素干扰效应  

Thyroid disrupting effects of perfluorooctane sulfonate on Xenopus laevis

作　　者：刘俊奇[1] 刘青坡[1] 曹庆珍[1] 钱丽娟[1] 郭素珍[1] 施华宏[1] 

机构地区：[1]华东师范大学环境科学系上海市城市化过程与生态恢复重点实验室,上海200062

出　　处：《华东师范大学学报（自然科学版）》2010年第4期118-124,共7页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(20877023);上海市自然科学基金(08ZR1406900);上海市城市化过程与生态恢复重点实验室开放基金(UPER0606);长江水环境教育部重点实验室开放基金(YRWEF06003)

摘　　要：运用两栖类变态实验（AMA）研究了全氟辛磺酸（PFOS）对于非洲爪蟾（Xenopuslaevis）蝌蚪的生长、变态和甲状腺组织结构的影响．结果显示，与对照组相比，7d后，10，50和250μμg·L^-1。PFOS暴露组蝌蚪的后肢长分别减小了17％，11％和18％，蝌蚪的发育延缓了1～2个阶段；19d后，10，50和250μg·L^-1PFOS组蝌蚪的体重分别增加了17％，10％和20％，体长分别增加了5％，4％和6％，而10μg·L^-1和250μg·L^-1。PFOS组蝌蚪的后肢长分别减小了12％和13％；暴露组甲状腺组织结构出现滤泡数目减少、上皮细胞增生、胶质减少甚至滤泡溶通等现象．结论是，在本实验浓度下，PFOS对于非洲爪蟾的蝌蚪生长有促进作用，对于变态发育过程却有抑制作用．参照AMA的方法，可以判断PFOS为抗甲状腺激素干扰物．The amphibian metamorphosis assay （AMA） was used to detect thyroid disrupting effects of perfluorooctane sulfonate （PFOS）. Xenopus laevis tadpoles at stage 51 were exposed to 10, 50 and 250 μg·L^-1 PFOS. The growth, metamorphosis and thyroid histology were observed. After 7 days of exposure, the hind limb l, engths were decreased by 11%- 18 %, and 1 or 2 developmental stages were delayed in treatment groups compared with the control. After 19 days of exposure, the body weights were increased by 10%-20%, the body lengths were increased by 4%-6%, and the hind limb lengths were decreased by 12%-13% in treatment groups. PFOS decreased the number of thyroid follicles, in which cell hyperplasia, colloid deple- tion and vaeuolation were observed. These results suggested that PFOS inhibited the develop- ment and increased the growth of X laevis. According to AMA, our results indicated that PFOS was an anti-thyroid hormone disrupting chemical.

关 键 词：甲状腺激素干扰物(TDCs) 非洲爪蟾(Xenopus laevis) 全氟辛磺酸(PFOS) 两栖类变态实验(AMA) 

分 类 号：Q948[生物学—植物学]