挂榜山小鲵线粒体DNA COⅠ基因及D-loop区序列研究  

Discussion on the Taxonomic Status of Hynobius guabangshanensis Based on Mitochondrial DNA COⅠ Gene and D-loop Region

作　　者：任巍[1] 牛艳东[2] 周毅[1] 王星[1] 邓学建[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081 [2]湖南省林业科学院,中国湖南长沙410004

出　　处：《生命科学研究》2010年第4期327-330,共4页Life Science Research

基　　金：湖南省科委自然科学基金资助项目(05JJ30068)

摘　　要：研究克隆了挂榜山小鲵COⅠ基因及D-loop区全长,结果显示挂榜山小鲵COⅠ基因及D-loop区全长分别为1 551 bp和803 bp.运用MEGA4.0软件P-distance法分别比较COⅠ基因及D-loop区小鲵科19个物种的遗传距离,结果显示挂榜山小鲵与小鲵属遗传距离比与小鲵科其它属物种的小,并且挂榜山小鲵与Zhang et al(2006)提交的中国小鲵相应序列的遗传距离几乎为0,构建了MP分子系统发生树也显示挂榜山小鲵与小鲵属同属一个分支,且与Zhang et al提交的中国小鲵聚为一小支.通过以上分析认为挂榜山小鲵属于小鲵科小鲵属,这与传统分类学方法一致.The complete sequence of COⅠand D-loop of Hynobius guabangshanensis were sequenced and the result showed that the length of COⅠis 1 551 bp and D-loop is 803 bp.Pairwise P-distance of COⅠ and D-loop sequences in 19 Hynobiidae species was calculated by MEGA4.0.Genetic distance of COⅠwas more stable than D-loop,and the result showed that Hynobius guabangshanensis belongs to Hynobius.Molecular phylogenetic tree of 19 Hynobiidae species was constructed using Maximum-parsimony methods.The MP trees also supported that Hynobius guabangshanensis belongs to Hynobius.The conclusion is the same with traditional methods of taxonomy.

关 键 词：挂榜山小鲵 COⅠ基因 D-LOOP区 分类地位 

分 类 号：Q959.52[生物学—动物学]