化毒胶囊对腺嘌呤所致慢性肾衰竭小鼠肾功能及病理形态学的影响  

Protective effects of Huadu capsule on adenine-induced chronic renal failure in mice

作　　者：高华[1] 张贺龙[2] 楚溪[3] 彭雯[4] 何士彦[4] 林悦[3] 孙振青[3] 张建平[3] 

机构地区：[1]河北省涿州市医院儿科,河北涿州072750 [2]河北省胸科医院骨科,河北石家庄050048 [3]河北医科大学第四医院药剂科,河北石家庄050011 [4]河北省石家庄市中心医院药剂科,河北石家庄050011

出　　处：《河北中医》2010年第7期1068-1070,共3页Hebei Journal of Traditional Chinese Medicine

摘　　要：目的观察化毒胶囊对腺嘌呤所致慢性肾衰竭(CRF)小鼠的保护作用。方法将40只雄性昆明种小鼠随机分为空白对照组、腺嘌呤模型组和化毒胶囊低、高剂量组,各10只。除空白对照组外,其余各组均按250mg/kg灌胃给予腺嘌呤,造成CRF动物模型,空白对照组予蒸馏水。将上述造模成功动物中的化毒胶囊低、高剂量组(分别0.6、1.2g/kg)均每日灌胃给药化毒胶囊1次,连续给药1个月;空白对照组与腺嘌呤模型组予蒸馏水。在给药1个月期间,除空白对照组外,其余各组每周予腺嘌呤(250mg/kg)1次,持续造成肾损害。测定各组小鼠体质量、肾质量、血肌酐(Cr)与尿素氮(BUN)含量,观察肾脏病理形态学变化。结果腺嘌呤模型组体质量、肾质量均低于空白对照组(P<0.05),Cr、BUN均高于空白对照组(P<0.05),说明造模成功。化毒胶囊高剂量组体质量高于腺嘌呤模型组(P<0.05),化毒胶囊低、高剂量组Cr、BUN均低于腺嘌呤模型组(P<0.05,P<0.01)。肾脏组织学观察可见肾小球病变、肾小管病变、间质内炎细胞浸润、管型等病理学改变。结论化毒胶囊对腺嘌呤所致CRF小鼠有保护作用。Objective To investigate the protective effects of Huadu capsule on renal function and renal pathological changes of chronic renal failure （CRF） induced by adenine in mice.Methods 40 Kunming mice were randomly divided into 4 groups：normal control group,model control group,high dose （1.2 g/kg） Huadu capsule group and low dose （0.6 g/kg） Huadu capsule groups.CRF was induced by oral administration of adenine （250 mg/kg） in mice.After establishment of model,mourse in high dose Huadu capsule groups were administrated with Huadu Capsule,normal control and model control groups were administrated with saline.The protective effects of Huadu capsule were observed by detection of renal function and pathology of renal tissue.Results The levels of blood urea nitrogen （BUN） and serum creatinin （SCR） in model group were higher than those in normal group （P〈0.05）,Huadu capsule could reversed the elevated levels of BUN and SCR （P〈0.05;P〈0.01）,improve the changes in pathology of renal tissue in CRF mice.Conclusion Huadu capsule can remarkably alleviate adenine-induced CRF in mice.

关 键 词：肾功能衰竭 慢性 小鼠模型 动物 腺嘌呤 胶囊 中药疗法 

分 类 号：R692.5[医药卫生—泌尿科学] R289.6[医药卫生—外科学]