粳型短光敏核不育系5021S育性基因的定位  

QTL Mapping for Fertility Gene of a New Short Photoperiod-sensitive Genic Male Sterile Japonica Rice Line 5021S

作　　者：黄胜东[1,2] 赵志刚[1] 江玲[1] 翟虎渠[3] 万建民[1,3] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程技术研究中心,江苏南京210095 [2]江苏省农业科学院,江苏省优质水稻工程技术研究中心,江苏南京210014 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《江苏农业学报》2010年第4期673-679,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑项目(2006BAD01A01-7);国家“863”项目(2010AA101302)

摘　　要：以育性转换表现为"长光高温下可育,短光低温下不育"反(向)光、温敏核不育种质5021S为材料,5021S与轮回422杂交组合F2作为遗传群体进行育性相关基因的QTL定位,利用124对SSR标记构建了一张全长1 912.3 cM平均图距为14.98 cM的饱和分子连锁图谱。利用Windows QTLCartographer2.5软件,在全基因组范围内检测到4个控制育性相关性状的QTLs位点,分别位于第2、第3、第5、第7染色体上,单个QTL可解释4.5%～32.5%表型变异。其中位于第2染色体上RM5804与RM425之间的qpms-2贡献率为32.5%,第3染色体RM130与RM3405之间的qpms-3贡献率为16.1%。表明5021S的核不育性受2对隐性主基因控制,同时受微效基因调控。Japonica rice,line 5012S,is a new short photoperiod sensitive genic male sterile one.The characteristics of fertility alterations in 5021S was opposite to the original P/TGMSR.It expresses fertility under the condition of long photoperiod and high temperature,and is sterile under the condition of short photoperiod and low temperature.A F2 population derived from the cross between 5021S and Lunhui422 was used to construct a genetic linkage map.Totally 124 SSR markers were mapped on 12 chromosomes covering 1 912.3 cM of rice genome.The average interval between these markers was 14.98 cM.Four QTLs were identified with significant additive effects on chromosomes 2,3,5 and 7.Each QTL explained 4.5%-32.5% of the phenotypic variance.The qpms-2 explained 32.5% phenotypic variance between RM5804 and RM425 on chromosome 2.The qpms-3 explained 16.1% phenotypic variance between RM130 and RM3405 on chromosome 3.The results indicated that male genic sterility of 5021S was controlled by two independent major recessive genes and some other minor genes.

关 键 词：粳稻 短光敏核不育 育性基因 QTL定位 

分 类 号：S511.22[农业科学—作物学]