扶正活血不同组方对肝纤维化大鼠肝组织α-平滑肌肌动蛋白和Smad3表达的影响  

Effects of different Fuzheng Huoxue prescription on expression of α-SMA and smad3 in liver fibrosis rats

作　　者：王者令 刘中景[1] 宋霆[1] 孙晓慧[1] 罗玮敏[1] 柳盛[1] 

机构地区：[1]青岛市传染病医院中西医结合肝病科,山东青岛266033

出　　处：《中西医结合肝病杂志》2010年第4期231-233,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：青岛市中医药科研计划重点资助课题(No.2009-ZYZ002)

摘　　要：目的:观察扶正活血不同组方对肝纤维化大鼠肝组织α-SMA(α-平滑肌肌动蛋白)、Smad3表达的影响。方法:SD大鼠90只随机分为6组,采用CCl4加橄榄油造摸,治疗组分为扶正活血1号方、2号方、3号方,并设阳性对照组、模型对照组和正常对照组,通过对各组动物灌服不同药物及生理盐水,分别检测其肝组织α-SMA和Smad3的表达水平。结果:扶正活血1号方和2号方较模型组和阳性对照组大鼠肝组织α-SMA表达产物减少,差异有显著性意义(P<0.01、P<0.05);而扶正活血3号方较模型组大鼠肝组织α-SMA表达均不明显,其差异无显著性意义。对于Smad3的影响,扶正活血1号方、2号方、3号方与模型组比较,差异均有显著性意义(P<0.05、P<0.01),其中1号方和2号方与阳性对照组比较,差异也有显著性意义(P<0.05、P<0.01),而3号方与阳性对照组比较,差异无显著性意义。结论:扶正活血1号方、2号方、3号方对大鼠肝组织α-SMA、Smad3表达均有显著影响。Objective：To investigate the effect of different Fuzheng Huoxu prescription on the expression of α-SMA and Smad3 in rat of liver fibrosis.Methods：Ninty SD rats were divided randomly in six groups.Liver fibrosis was established by carbon tetrachloride （CCl4） plus olive oil treatment.Therapeutic groups include No.1 Fuzheng Huoxue,No.2 Fuzheng Huoxue and No.3 Fuzheng Huoxue,along with the positive control group,model control group and normal control group.The expression levels of α-SMA and Smad3 from the liver tissue were detected after the rat of liver fibrosis treated with different medicines and physiological saline.Results：The expression levels of α-SMA were significantly lower in No.1 and No.2 Fuzheng Huoxue groups than model control and positive control groups（P〈0.01,P〈0.05） while there was no obvious difference in the expression level of αSMA between the No.3 Fuzheng Huoxue and model control groups.The expression levels of Smad3 were significantly lower in all the three Fuzheng Huoxue medicine groups than that in model control group（P〈0.05,P〈0.01）.Also,The expression levels of Smad3 were significantly lower in No.1 and No.2Fuzheng Huoxue medicine groups when compared with that in positive control group（P〈0.05,P〈0.01） while no obvious difference was observed between the No.3 Fuzheng Huoxue medicine and positive control groups.Conclusion：Different Fuzheng Huoxue prescription have the inhibitory effects on the expression of α-SMA and Smad3.

关 键 词：扶正活血不同组方/药理作用 Α-平滑肌肌动蛋白 SMAD3 肝纤维化 大鼠 

分 类 号：R285.5[医药卫生—中药学]