阿仑膦酸钠预防卵巢切除大鼠腰椎间盘退变  

Alendronate prevents lumbar intervertebral disc degeneration in ovariectomized rats

作　　者：李震[1] 张柳[1] 穆树林[1] 李强[1] 程潭[1] 田发明[1] 

机构地区：[1]华北煤炭医学院附属医院骨科,唐山063000

出　　处：《解剖学杂志》2010年第4期464-467,505,共5页Chinese Journal of Anatomy

摘　　要：目的： 探讨阿仑膦酸钠对卵巢切除大鼠腰椎间盘退变预防作用及其作用机制.方法： SD大鼠随机均分为Sham组,双侧卵巢切除＋盐水组（OVX＋V组）,双侧卵巢切除＋阿仑膦酸钠（OVX＋ALN组）.处死前10d和4d分别给予四环素和钙黄绿素双荧光标记.取腰2椎体进行骨形态计量学分析,腰4～5腰椎及椎间盘进行VG特殊染色,免疫组织化学检测椎间盘基质金属蛋白酶-13（MMP-13）的表达,观察椎间盘的病理学改变并根据组织学评分系统对腰椎间盘的退变程度（LVD）进行评分.结果： 腰椎骨量OVX＋V组低于Sham组及OVX＋ALN组;腰椎间盘评分OVX＋V组高于Sham组及OVX＋ALN组; OVX＋V组大鼠椎间盘中MMP-13表达高于OVX＋ALN组、Sham组.结论：阿仑膦酸钠可预防卵巢切除大鼠腰椎间盘的退变,其作用可能与维持椎体骨量及微观结构,降低椎间盘中MMP13的表达,抑制基质的降解有关.Objective： To investigate the effects of alendronate （ALN） on lumbar intervertebral disc degeneration （LVD） in the ovariectomy （OVX） rats. Methods： Thirty 3-month old female Sprague-Dawley rats were randomized into three groups （n=10）. An ovariectomy was performed in 20 rats and the other 10 rats was subjected to a sham surgery. Rats in ALN group were subcutaneous injection with ALN （20μg/kg/2d） for 12 weeks. All rats were sacrificed at 12 weeks after surgery. Double labeling was performed with subcutaneous injections of tetracylcin 10 days and of calcein 4 days before sacrifice. Bone histomorphometric analysis was applied to the L2 lumbar spines in all rats. L4 L5 lumbar spines and intervertebral disc sections were stained by collagen VG staining, and immunostained for matrix metatloproteinase 13 （MMP-13）. The pathological changes of intervertebral discs were observed and degree of LVD was scored by a histological scoring system. Results： Spine bone mass in the OVX＋V group were significantly lower than that in the sham group and the OVX＋ALN group. Rats in the OVX＋V group had significantly higher score than those in the sham group and the OVX＋ALN group. The expression level of MMP-13 in the OVX＋V group was significantly higher than that in the sham group and the OVX＋ALN group. Conclusion： ALN could suppress lumbar intervertebral disc degeneration in the OVX rats, preventing the bone loss and deterioration of microarchitecture of lumbar vertebrae as well as inhibiting the degradation of matrix by downregulation of MMP-13.

关 键 词：卵巢切除 椎间盘退变 免疫组织化学 阿仑膦酸钠 基质金属蛋白酶-13 

分 类 号：R681.53[医药卫生—骨科学]