I型星形胶质细胞条件培养液抑制H2O2诱导PCl2细胞凋亡机制  

Mechanism of inhibition of typeⅠ astrocytes-conditioned medium on apoptosis of PC12 induced by H2O2

作　　者：赵丽娟[1] 李滢[1] 汪润[1] 孙茂民[1] 

机构地区：[1]苏州大学医学部解剖学神经细胞生物学研究室,苏州215123

出　　处：《解剖学杂志》2010年第4期498-501,共4页Chinese Journal of Anatomy

摘　　要：目的： 探讨Ⅰ型星形胶质细胞条件培养液（ACM-1）对H2O2诱导PC12细胞凋亡的抑制作用与硫氧还蛋白（Trx）表达的关系.方法： 采用MTT法检测ACM-1预处理H2O2诱导PC12细胞的活力.流式细胞术,Real time-PCR法及免疫印迹法分别检测细胞凋亡率,Trx-1和Trx-2mRNA的变化及Trx和Bcl-2蛋白水平的变化.结果： 20%浓度的ACM-1能提高H2O2诱导PC12细胞的活力,细胞凋亡率下降,Trx-1的mRNA量升高,Trx和Bcl-2的蛋白量增加.结论： ACM-1对H2O2诱导PC12细胞凋亡的抑制作用可能是通过Trx上调介导的,而Trx的抗凋亡作用和Bcl-2的上调有关.Objective： To study inhibition of the type I astrocytes conditioned medium （ACM-1） on apoptosis of PC12 induced by H2O2 and the expression level of thioredoxin （Trx） protein. Methods： MTT assay was perfonmed to detect the viability of type I astrocytes-conditioned medium on PC12 induced by H2O2. Flow cytometry, real time-PCR and Western blotting were employed to measure apoptosis rate of cells, mRNA of Trx-1 and Try2, and the expression level of Trx protein and Bcl-2 protein respectively. Results： 20% of ACM-1 increased the viability of PC12 induced by H2O2. The apoptotic rate was significantly decreased compared with the control group. The expresstions of Trx-1 mRNA, Trx and Bcl-2 protein were significantly increased compared with the control group. Condusion： The inhibition effect of type I astrocytes conditioned medium on the apoptosis of PC12 induced by H2O2 is mediated, at least in part, by the up-regulation of Trx expresstion. The antiapoptosis of Trx is correlated with the up-regulated expression of Bcl-2 protein.

关 键 词：Ⅰ型星形胶质细胞 条件培养液 硫氧还蛋白 PC12 细胞 凋亡 

分 类 号：R392.2[医药卫生—免疫学]